2010 Fiscal Year Final Research Report
Isolation of oral cancer stem cells and development of new therapies targeted cancer stem cells.
| Project/Area Number |
20390517
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIRASUNA Kanemitsu Kyushu University, 医歯薬学総合研究科, 特任教授 (30093420)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIURA Tsuyoshi 九州大学, 大学病院, 講師 (40322292)
IKARI Tatsuya 九州大学, 大学病院, 助教 (70380467)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 臨床腫瘍学 |
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| Research Abstract |
To analyze the mechanisms of adenoid cystic carcinoma (AdCC) metastasis, we established green fluorescence protein (GFP)-transfected parental ACCS-GFP and high metastatic ACCS-M GFP. ACCS-M GFP displayed sphere-forming ability and high expression of epithelial- mesenchymal transition (EMT)-related genes, stem cell and differentiation markers. Transfection with short hairpin RNA (shRNA) silencing of the T-box transcription factor Brachyury (also a differentiation marker) resulted in downregulation of the EMT and stem cell markers. In addition, sphere-forming ability and EMT characteristics were simultaneously lost.
We conclude that the EMT is directly linked to CSC and that Brachyury is a central regulator of EMT and CSC. These results suggest that Brachyury will be a potential therapeutic target for anti-CSC therapy for AdCC in the future.
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