Elucidation of onset mechanism of amelogenesis imperfecta (AI) using a biomineralization model

2010 Fiscal Year Final Research Report

• Project/Area Number: 20390522
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KOTARO Tanimoto Hiroshima University, 病院, 講師 (20322240)
• Co-Investigator(Kenkyū-buntansha): TANNE Kazuo 広島大学, 大学院・医歯薬学総合研究科, 教授 (30159032) ; NOBUAKI Tanaka 広島大学, 大学院・医歯薬学総合研究科, 助教 (90397969)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Eiji 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (40273693)
• Project Period (FY): 2008 – 2010
• Keywords: エナメル蛋白 / バイオミネラリゼーション / エナメル質形成不全症 / アメロゲニン / ハイドロキシアパタイト

Research Abstract

The purpose of this study was to investigate the effects of mutation of amelgenin, an enamel matrix protein, on the enamel crystal formation, and to elucidate of onset mechanism of amelogenesis imperfecta (AI).
Wild-type recombinant human full-length amelogenin and mutated recombinant human full-length ameogenin (P41T) were synthesized. There was a difference in the aggregation ability in solution at neutral pH (pH 7.4) between the wild-type and mutated full-length amelogenins. In addition, recombinant human MMP20 wa synthesized, and the digestion of recombinant human full-length amelogenin by the MMP20 was investigated. The digestion of mutated recombinant human full-length amelogenin was delayed as compared with that in wild-type amelogenin.
From these results, it was strongly suggested that a mutation of amelogenin may induce the abnormality of enamel structure during tooth formation.

Research Products

• [Journal Article] Amelogenin enhances the proliferation of cementoblast lineage cells (2011)
  • Author(s): Kunimatsu R, Tanimoto K, Tanne Y, Kamiya T, Ohkuma S, Huang YC, Yoshimi Y, Miyauchi M, Takata T, Tanne K.
  • Journal Title: J Periodontol in press.
  • Peer Reviewed
• [Journal Article] Effects of human full-length amelogenin on the proliferation of human mesenchymal stem cells derived from bone marrow. (2010)
  • Author(s): Huang YC, Tanimoto K, Tanne Y, Kamiya T, Kunimatsu R, Michida M, Yoshioka M, Yoshimi Y, Kato Y, Tanne K
  • Journal Title: Cell Tissue Res. 342(2) Pages: 205-212
  • Peer Reviewed
• [Journal Article] Functional roles of prolines at amelogenin C terminal during tooth enamel formation. (2009)
  • Author(s): Zhu L, Tanimoto K, Le T, DenBesten PK, Li W
  • Journal Title: Cells Tissues Organs. 189(1-4) Pages: 203-206
  • Peer Reviewed
• [Journal Article] Comparative properties of recombinant human and bovine matrix metalloproteinase-20 (2008)
  • Author(s): Zhu L, Tanimoto K, Robinsin S, Chen J, Witkowska E, Hall S, Le T, Denbesten PK, Li W
  • Journal Title: Arch Oral Biol. 53(8) Pages: 785-790
  • Peer Reviewed
• [Journal Article] Effects of fluoride on the interactions between amelogenin and apatite crystals. (2008)
  • Author(s): Tanimoto K, Le T, Zhu L, Chen J, Featherstone JD, Li W, DenBesten P
  • Journal Title: J Dent Res. 87(1) Pages: 39-44
  • Peer Reviewed
• [Journal Article] Reduced amelogenin-MMP20 interactions in amelogenesis imperfecta. (2008)
  • Author(s): Tanimoto K, Le T, Zhu L, Witkowska HE, Robinson S, Hall S, Hwang P, Denbesten P, Li W
  • Journal Title: J Dent Res. 87(5) Pages: 451-455
  • Peer Reviewed
• [Presentation] 歯周組織構成細胞に対するヒトリコンビナントアメロゲニンの効果とシグナル伝達機構の解明 (2010)
  • Author(s): 國松亮, 谷本幸太郎, 丹根由起, 神谷貴志, 大熊暁, 岩渕泰憲, 黄郁〓, 吉岡基子, 尾崎徳継, 笹本智子, 道田将彦, 廣瀬尚人, 光吉智美, 吉見友希, 丹根一夫
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: Yokohama
  • Year and Date: 20100927-20100929
• [Presentation] Effecs of amelogenin on the proliferation of mesenchymak stem cells (2010)
  • Author(s): 黄郁〓, 谷本幸太郎, 丹根由起, 神谷貴志, 犬伏俊博, 吉岡基子, 道田将彦, 笹本智子, 吉見友希, 岩渕泰憲, 丹根一夫
  • Organizer: The 88^<th> International Association for Dental Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20100714-20100717
• [Presentation] Amelogenin Modulates the Proliferation of Human Mesenchymal Stem Cells through the MAPK-ERK Pathway (2010)
  • Author(s): Huang YC, Tanimoto K, Tanne Y, Kamiya T, Ohkuma S, Inubushi T, Kunimatsu R, Yoshioka M, Michida M, Mitsuyoshi T, Yoshimi Y, Kato Y, Tanne K
  • Organizer: The 2^<nd> International Conference on Orthodontic Treatment
  • Place of Presentation: Hiroshima
  • Year and Date: 20100403-20100404
• [Presentation] Effects of recombinant human amelogenin on the proliferation of cementoblasts and periodontal ligament cells (2010)
  • Author(s): 國松亮, 谷本幸太郎, 丹根由起, 神谷貴志, 大熊暁, 犬伏俊博, 黄郁〓, 吉岡基子, 廣瀬尚人, 光吉智美, 吉見友希, 宮内睦美, 高田隆, 丹根一夫
  • Organizer: The International Workshop on BioDental Education & Research 2010
  • Place of Presentation: Hiroshima
  • Year and Date: 20100211-20100212
• [Presentation] ヒト歯根膜細胞代謝調節機構に対するヒトリコンビナントアメロジェニンの効果 (2009)
  • Author(s): 国松亮、谷本幸太郎、田中伸明、丹根由起、神谷貴志、大熊暁、犬伏俊博、岩渕泰憲、黄郁〓、吉岡基子、尾崎徳継、笹本智子、道田将彦、光吉智美、丹根一夫
  • Organizer: 第68回日本矯正歯科学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20091117-20091119
• [Presentation] Effects of recombinant human amelogenin on the proliferation of cementoblasts and periodontal ligament cells (2009)
  • Author(s): 国松亮, 谷本幸太郎, 田中伸明, 丹根由起, 神谷貴志, 大熊暁, 犬伏俊博, 黄郁〓, 吉岡基子, 廣瀬尚人, 光吉智美, 吉見友希, 宮内睦美, 高田隆, 丹根一夫
  • Organizer: 3rd Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 20091107-20091108
• [Presentation] ヒトセメント芽細胞の代謝に対するヒトリコンビナントアメロジェニンの影響 (2009)
  • Author(s): 国松亮、谷本幸太郎、田中伸明、丹根由起、神谷貴志、大熊暁、黄郁〓、吉岡基子、丹根一夫
  • Organizer: 第67回日本矯正歯科学会学術大会
  • Place of Presentation: 千葉市
  • Year and Date: 20090916-20090918
• [Presentation] Effects of Amelogenin on the Proliferation and Differentiation of Human Mesenchymal Stem Cells (2009)
  • Author(s): Huang YC, Tanimoto K, Tanaka N, Tanne Y, Kamiya T, Kunimatsu R, Yoshioka M, Michida M, Kato Y, Tanne K
  • Organizer: Workshop on Bio-Dental Education and Research in Hiroshima
  • Place of Presentation: Hiroshima
  • Year and Date: 20090207-20090208
• [Presentation] アメロジェニンのセメント質代謝調節機構への影響 (2009)
  • Author(s): 国松亮, 谷本幸太郎, 田中伸明, 丹根由起, 神谷貴志, 大熊暁, 黄郁〓, 吉岡基子, 光吉智美, 宮内睦美, 高田隆, 丹根一夫
  • Organizer: 第42回広島大学歯学会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-20
• [Presentation] Amelogenesis Imperfecta Is Related to Decreased Amelogenin-MMP20 Interactions (2008)
  • Author(s): Li W, Tanimoto K, Zhu L, Le T, Habelitz S, DenBesten PK
  • Organizer: 86th General Session & Exhibition of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20080702-20080705
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] エナメル質再生液及びエナメル質再生キット (2011)
  • Inventor(s): 谷本幸太郎、丹根一夫、国松亮、神谷貴志
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 特許,特願2011-027923
  • Filing Date: 2011-02-10