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2010 Fiscal Year Final Research Report

Molecular dissection of membrane traffic machinery for innate immunity

Research Project

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Project/Area Number 20390528
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Periodontal dentistry
Research InstitutionOsaka University

Principal Investigator

AMANO Atsuo  Osaka University, 大学院・歯学研究科, 教授 (50193024)

Co-Investigator(Kenkyū-buntansha) KAWAI Shinji  大阪大学, 大学院・歯学研究科, 助教 (40362678)
KATO Takahiro  大阪大学, 大学院・歯学研究科, 助教 (20423139)
FURUTA Nobumichi  大阪大学, 大学院・歯学研究科, 助教 (50452446)
Co-Investigator(Renkei-kenkyūsha) YOSHIMORI Tamotsu  大阪大学, 大学院・医学研究科, 教授 (60191649)
Project Period (FY) 2008 – 2010
Keywords歯学 / 歯周病菌 / 歯周病 / 感染症 / シグナル伝達 / 細菌・組織
Research Abstract

Porphyromonas gingivalis, the most well-known periodontal pathogen, can enter gingival epithelial cells and pass through the epithelial barrier into deeper tissues. However, it is poorly understood how this pathogen exits from infected cells for further transcellular spreading. The present study was performed to elucidate the cellular machinery exploited by P. gingivalis to exit from immortalized human gingival epithelial cells. P. gingivalis was internalized with early endosomes, and about half of the intracellular bacteria were found to be sorted to lytic compartments, including autolysosomes and late endosomes/lysosomes, while a considerable number of the remaining organisms were sorted to recycling endosomes. Small GTpases (Rab11 and RalA) and exocyst complex subunits (Sec5, Sec6, and Exo84) were also shown to be involved in the exit of P. gingivalis. The recycling pathway is exploited by intracellular P. gingivalis to exit from infected cells to neighboring cells as a mechanism of cell-to-cell spreading.

  • Research Products

    (44 results)

All 2011 2010 2009 2008 Other

All Journal Article (28 results) (of which Peer Reviewed: 28 results) Presentation (11 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.2011

    • Author(s)
      Takeuchi H, Furuta N, Morisaki I, Amano A
    • Journal Title

      Cellular Microbiology in press

    • Peer Reviewed
  • [Journal Article] Odd-skipped related 2 regulates genes related to proliferation and development.2010

    • Author(s)
      Kawai S, Abiko Y, Amano A
    • Journal Title

      Biochemical and Biophysical Research Communications 398(2)

      Pages: 184-190

    • Peer Reviewed
  • [Journal Article] Odd-skipped related 2 is epigenetically regulated in cellular quiescence.2010

    • Author(s)
      Kawai S, Amano A
    • Journal Title

      Biochemical and Biophysical Research Communications 396(4)

      Pages: 831-836

    • Peer Reviewed
  • [Journal Article] Combinational soluble N-ethylmaleimide-sensitive actor attachment protein receptor proteins VAMP8 and Vti1b mediate the fusion of antibacterial and canonical autophagosomes with lysosomes.2010

    • Author(s)
      Furuta N, Fujita N, Noda T, Yoshimori T, Amano A
    • Journal Title

      Molecular Biology of the Cell 21(6)

      Pages: 1001-1010

    • Peer Reviewed
  • [Journal Article] Host-parasite interactions in periodontitis : Microbial pathogenicity and innate immunity.2010

    • Author(s)
      Amano A
    • Journal Title

      Periodontology 2000 54

      Pages: 9-14

    • Peer Reviewed
  • [Journal Article] Genotyping to distinguish microbial pathogenicity in periodontitis.2010

    • Author(s)
      Kuboniwa M, Inaba H, Amano A
    • Journal Title

      Periodontology 2000 54

      Pages: 136-159

    • Peer Reviewed
  • [Journal Article] Outer membrane vesicles function as offensive weapons in host-parasite interactions.2010

    • Author(s)
      Amano A
    • Journal Title

      Microbes and Infection 12(11)

      Pages: 791-798

    • Peer Reviewed
  • [Journal Article] Cellular machinery to fuse antimicrobial autophagosome with lysosome.2010

    • Author(s)
      Furuta N, Amano A
    • Journal Title

      Communicative & Integrative Biology 3(4)

      Pages: 385-387

    • Peer Reviewed
  • [Journal Article] Roles of oral bacteria in cardiovascular diseases-From molecular mechanisms to clinical cases : Implication of periodontal diseases in development of systemic diseases.2010

    • Author(s)
      Inaba H, Amano A
    • Journal Title

      Journal of Pharmacological Sciences 113(2)

      Pages: 103-109

    • Peer Reviewed
  • [Journal Article] Mediatory molecules that fuse autophagosomes and lysosomes.2010

    • Author(s)
      Furuta N, Yoshimori T, Amano A
    • Journal Title

      Autophagy 6(3)

      Pages: 417-418

    • Peer Reviewed
  • [Journal Article] Host-parasite interactions in periodontitis: Subgingival infection and host sensing.2010

    • Author(s)
      Amano A
    • Journal Title

      Periodontology 2000 52

      Pages: 7-11

    • Peer Reviewed
  • [Journal Article] Bacterial adhesions to host components in periodontitis.2010

    • Author(s)
      Amano A
    • Journal Title

      Periodontology 2000 52

      Pages: 12-37

    • Peer Reviewed
  • [Journal Article] Host membrane trafficking for conveyance of intracellular oral pathogens.2010

    • Author(s)
      Amano A
    • Journal Title

      Periodontology 2000 52

      Pages: 84-93

    • Peer Reviewed
  • [Journal Article] Entry of Porphyromonas gingivalis outer membrane vesicles into epithelial cells causes cellular functional impairment.2009

    • Author(s)
      Furuta N, Takeuchi H, Amano A
    • Journal Title

      Infection and Immunity 77(11)

      Pages: 761-4770

    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis invades human trophoblasts and inhibits proliferation by inducing G1 arrest and apoptosis.2009

    • Author(s)
      Inaba H, Kuboniwa M, Bainbridge B, Yilmaz O, Katz J, Shiverick KT, Amano A
    • Journal Title

      Cellular Microbiology 11(10)

      Pages: 1517-1532

    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis outer membrane vesicles enter human epithelial cells via an endocytic pathway and are sorted to lysosomal compartments.2009

    • Author(s)
      Furuta N, Tsuda K, Omori H, Yoshimori T, Yoshimura F, Amano A
    • Journal Title

      Infection and Immunity 77(10)

      Pages: 4187-4196

    • Peer Reviewed
  • [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations.2009

    • Author(s)
      KuboniwaM, Aman A
    • Journal Title

      Oral Microbiology and Immunology 24(3)

      Pages: 260-263

    • Peer Reviewed
  • [Journal Article] Upregulation of S100 calcium-binding protein A9 is required for induction of smooth muscle cell proliferation by a periodontal pathogen.2009

    • Author(s)
      Inaba H, Hokamura K, Nakano K, Nomura R, Katayama K, Nakajima A, Yoshioka Y, Taniguchi K, Kamisaki Y, Ooshima T, Umemura K, Murad F, Wada K, Amano A
    • Journal Title

      FEBS Letters 583(1)

      Pages: 128-134

    • Peer Reviewed
  • [Journal Article] Group A Streptococcus. A loser in the battle with autophagy.2009

    • Author(s)
      Yoshimori T, Amano A
    • Journal Title

      Current Topics in Microbiology and Immunology 335

      Pages: 217-226

    • Peer Reviewed
  • [Journal Article] Odd-skipped related 1 gene expression is regulated by Runx2 and Ikzf1 transcription factors.2008

    • Author(s)
      Yamauchi M, Kawai S, Kato T, Ooshima T, Amano A
    • Journal Title

      Gene 426

      Pages: 81-90

    • Peer Reviewed
  • [Journal Article] Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment.2008

    • Author(s)
      Makiura N, Ojima M, Kou Y, Furuta N, Okahashi N, Shizukuishi S, Amano A
    • Journal Title

      Oral Microbiology and Immunology 23(4)

      Pages: 348-351

    • Peer Reviewed
  • [Journal Article] Signaling pathways in osteoblast proinflammatory responses to infection by Porphyromonas gingivalis.2008

    • Author(s)
      Ohno T, Okahashi N, Morisaki I, Amano A
    • Journal Title

      Oral Microbiology and Immunology 23(2)

      Pages: 96-104

    • Peer Reviewed
  • [Journal Article] Porphyromonas gingivalis gingipains cause G1 arrest in osteoblastic/stromal cells.2008

    • Author(s)
      Kato T, Tsuda T, Kawai S, Inaba H, Okahashi N, Shibata Y, Abiko Y, Amano A
    • Journal Title

      Oral Microbiology and Immunology 23(2)

      Pages: 158-164

    • Peer Reviewed
  • [Journal Article] Identification of hop polyphenolic components which inhibit prostaglandin E2 production by gingival epithelial cells stimulated with periodontal pathogen.2008

    • Author(s)
      Inaba H, Tagashira M, Honma D, Kanda T, Kou Y, Ohtake Y, Amano A
    • Journal Title

      Biological & Pharmaceutical Bulletin 31(3)

      Pages: 527-530

    • Peer Reviewed
  • [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae.2008

    • Author(s)
      Inaba H, Nakano K, Kato T, Nomura R, Kawai S, Kuboniwa M, Ishihara K, Ooshima T, Amano A
    • Journal Title

      Oral Microbiology and Immunology 23(1)

      Pages: 29-35

    • Peer Reviewed
  • [Journal Article] Inflammatory responses of gingival epithelial cells stimulated with Porphyromonas gingivalis vesicles are inhibited by hop-associated polyphenols.2008

    • Author(s)
      Kou Y, Inaba H, Kato T, Tagashira M, Honma D, Kanda T, Ohtake Y, Amano A
    • Journal Title

      Journal of Periodontology 79(1)

      Pages: 174-180

    • Peer Reviewed
  • [Journal Article] Functional analysis of alpha5beta_1 integrin and lipid rafts in invasion of epithelial cells by Porphyromonas gingivlis using fluorescent beads coated with bacterial membrane vesicles.2008

    • Author(s)
      Tsuda K, Furuta N, Inaba H, Kawai S, Hanada K, Yoshimori T, Amano A
    • Journal Title

      Cell Structure and Function 33(1)

      Pages: 123-132

    • Peer Reviewed
  • [Journal Article] Etiologic factors of early-onset periodontal disease in Down syndrome.2008

    • Author(s)
      Amano A
    • Journal Title

      Japanese Dental Science Review 44(2)

      Pages: 118-127

    • Peer Reviewed
  • [Presentation] 歯周病菌と4つの全身疾患シンポジウム「ここまでわかった口腔細 菌による全身疾患:最新の研究と臨床知見」2010

    • Author(s)
      天野敦雄、稲葉裕明
    • Organizer
      第31回日本臨床薬理学会年会
    • Place of Presentation
      京都市
    • Year and Date
      2010-12-03
  • [Presentation] 上皮細胞への細菌侵入とメンブレント ラッフィク サテライトシンポジウム「歯学領域にお けるメンブレントラフィック研究」2010

    • Author(s)
      天野敦雄、竹内洋輝、古田信道
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      千葉
    • Year and Date
      2010-09-20
  • [Presentation] Systemic diseases and periodontal pathogen.2010

    • Author(s)
      天野敦雄
    • Organizer
      Leeds & Osaka Universities International Symposium in Dentistry
    • Place of Presentation
      Leeds, England
    • Year and Date
      2010-07-05
  • [Presentation] 細胞内ロジスティクス機構による細菌感染症の制御2010

    • Author(s)
      天野敦雄、竹内洋輝、古田信道
    • Organizer
      新学術領域「細胞内ロジスティクス」班会議
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-30
  • [Presentation] Intracellular Localization of Porphyromonas gingivalis.2009

    • Author(s)
      竹内洋輝, 古田信道, 天野敦雄
    • Organizer
      Kyungpook-Osaka University International Symposium in Dentistry
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2009-12-11
  • [Presentation] 細胞内ロジスティクス機構による細菌 感染症の制御2009

    • Author(s)
      天野敦雄、古田信道
    • Organizer
      The 1st Conference on Intracellular Logistics
    • Place of Presentation
      沖縄県国頭郡恩納村
    • Year and Date
      2009-11-11
  • [Presentation] Porphyromonas gingivalisの侵入による細 胞傷害と全身疾患の関連2009

    • Author(s)
      天野敦雄、古田信道、稲葉裕明
    • Organizer
      学術フロンティア推進事業シンポジウム
    • Place of Presentation
      千葉県松戸市
    • Year and Date
      2009-10-03
  • [Presentation] Outer membrane vesicles are "bombs" of Porphyromonas gingivali s in periodontitis.2009

    • Author(s)
      天野敦雄
    • Organizer
      Korea-Japan Symposium for Dentistry
    • Place of Presentation
      Daegu, Korea
    • Year and Date
      2009-06-02
  • [Presentation] 長寿を支える歯の健康2009

    • Author(s)
      天野敦雄
    • Organizer
      厚生労働省長寿総合研究事業公開市民講座「高齢者とお口の健康管理 ~ いつまでも美味しく食べるために~」
    • Place of Presentation
      大阪
    • Year and Date
      2009-03-22
  • [Presentation] 稲葉裕明歯周病菌:歯垢から全身疾患へ「循環器系全身疾患における口腔細菌の役割:分子メカニズムから臨床知見まで」2009

    • Author(s)
      天野敦雄
    • Organizer
      第82回日本薬理学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-03-16
  • [Presentation] Outer membrane vesicles are "Bombs" of Porphyromonas gingivalis.2008

    • Author(s)
      天野敦雄
    • Organizer
      Sino-Japanese Symposium of Conservative Dentistry
    • Place of Presentation
      Chendu, China
    • Year and Date
      2008-09-09
  • [Book] 「カラーアトラス口腔嫌気性グラム陰性桿菌」病原菌の今日的意味(4版松本慶蔵編),大阪2011

    • Author(s)
      天野敦雄, 浜田茂幸
    • Publisher
      医薬ジャーナル(印刷中)
  • [Book] 「口腔嫌気性グラム陰性桿菌:歯周病を惹起するプラーク細菌」病原菌の今日的意味(4版松本慶蔵編),大阪2011

    • Author(s)
      天野敦雄, 浜田茂幸
    • Publisher
      医薬ジャーナル(印刷中)
  • [Book] 「歯周病研究のフロンティア:歯周病細菌から組織再生まで」生命歯科医学のカッティングエッジ(米田俊之編),大阪2008

    • Author(s)
      天野敦雄, 加藤隆大, 稲葉裕明, 河合伸治, 岡橋暢夫
    • Total Pages
      98-108
    • Publisher
      大阪大学出版会
  • [Remarks] ホームページ

    • URL

      http://www.dent.osaka-u.ac.jp/~methodol/

  • [Patent(Industrial Property Rights)] ホップ製剤の製造方法、ホップ製剤、抗炎症剤、飲食品および口腔用品2010

    • Inventor(s)
      稲葉裕明、本間大樹、田頭素行、神田智正、天野敦雄
    • Industrial Property Rights Holder
      アサヒビール株式会社
    • Industrial Property Number
      特許JP2007066148
    • Filing Date
      2010-01-26

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

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