The study on mycotoxin detoxification and protection of animal mycotoxicoses in Thaoland.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20405042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: The University of Tokyo
• Principal Investigator: KUMAGAI Susumu The University of Tokyo, 大学院・農学生命科学研究科, 教授 (60109965)
• Co-Investigator(Kenkyū-buntansha): KONISHI Yoshiko 国立医薬品食品衛生研究所, 衛生微生物部, 部長 (10195761) ; SAKUDA Shohei 東京大学, 大学院・農学生命科学研究科, 准教授 (80192087)
• Co-Investigator(Renkei-kenkyūsha): TAKATORI Kousuke NPO法人カビ相談センター, 理事長 ; PRAPEUK Tangmunkhong Kasetsart University, Faculty of Veterinary Medicine, Associate Professor ; AMNART Poapolathep Kasetsart University, Faculty of Veterinary Medicine, Associate Professor
• Project Period (FY): 2008 – 2010
• Keywords: アフラトキシン / ゼアラレノン / タイ / 家畜 / 分解

Research Abstract

In order to find metabolic detoxification by micro-organisms in soil of Thailand, where heavy contamination with mycotoxins has been observed, bacteria and fungi isolated from Cassava farms in Thailand were examined for their activities to metabolize aflatoxin, ochratoxin A and zearalenone. The result demonstrated some bacteria and fungi to metabolize aflatoxin B1 and ochratoxin A. Feces of horse, sheep and pig living in the central region of Thailand, and anaerobic bacteria isolated from the feces, were also examined for their metabolic activities toward mycotoxins, but clear metabolism was not found. With regard to animal tissues, a possible conversion of aflatoxin B2 to B1 by the liver tissues from various animals was suggested. Also tissues of various organs of goat were demonstrated to metabolize zearalenone to alpha-and beta-zearalenols.

Research Products

• [Journal Article] Simulation of deoxynivalenol intake from wheat consumption in Japan using the Monte Carlo method. (2011)
  • Author(s): Nakatani Y, Satoh T, Saito S, Watanabe M, Yoshiike N, Kumagai S, Sugita-Konishi Y
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 28(4) Pages: 471-6
  • Peer Reviewed
• [Journal Article] Rapid deposition of glomerular IgA in BALB/c mice by nivalenol and its modifying effect on high IgA strain (HIGA) mice. (2011)
  • Author(s): Dewa Y, Kemmochi S, Kawai M, Saegusa Y, Harada T, Shimamoto K, Mitsumori K, Kumagai S, Sugita-Konishi Y, Shibutani M
  • Journal Title: Exp Toxicol Pathol. 63(1-2) Pages: 17-24
  • Peer Reviewed
• [Journal Article] Enumeration of Fungi in Fruits by the Most Probable Number Method. (2010)
  • Author(s): Watanabe M, Tsutsumi F, Lee KI, Sugita-Konishi Y, Kumagai S, Takatori K, Hara-Kudo Y, Konuma H
  • Journal Title: J Food Sci. 75(9) Pages: M564-M567
  • Peer Reviewed
• [Journal Article] Rapid and effective DNA extraction method with bead grinding for a large amount of fungal DNA. (2010)
  • Author(s): Watanabe M, Lee K, Goto K, Kumagai S, Sugita-Konishi Y, Hara-Kudo Y
  • Journal Title: J Food Prot. 73(6) Pages: 1077-84
  • Peer Reviewed
• [Journal Article] The effect of naringenin on the fate and disposition of deoxynivalenol in piglets. (2010)
  • Author(s): Poapolathep A, Poapolathep S, Sugita-Konishi Y, Wongpanit K, Machii K, Itoh Y, Kumagai S
  • Journal Title: J Vet Med Sci. 72(10) Pages: 1289-94
  • Peer Reviewed
• [Journal Article] Exposure to aflatoxins in Japan : risk assessment for aflatoxin B1. (2010)
  • Author(s): Sugita-Konishi Y, Sato T, Saito S, Nakajima M, Tabata S, Tanaka T, Norizuki H, Itoh Y, Kai S, Sugiyama K, Kamata Y, Yoshiike N, Kumagai S
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 27(3) Pages: 365-72
  • Peer Reviewed
• [Journal Article] Four-year surveillance for ochratoxin a and fumonisins in retail foods in Japan. (2010)
  • Author(s): Aoyama K, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Fujita K, Kai S, Tsutsumi T, Takahashi M, Tanaka H, Iizuka S, Ogiso M, Maeda M, Yamaguchi S, Sugiyama K, Sugita-Konishi Y, Kumagai S.
  • Journal Title: J Food Prot. 73(2) Pages: 344-52
  • Peer Reviewed
• [Journal Article] Metabolic conversion of zearalenone to alpha-zearalenol by goat tissues. (2010)
  • Author(s): Dong M, Tulayakul P, Li JY, Dong KS, Manabe N, Kumagai S.
  • Journal Title: J Vet Med Sci. 72(3) Pages: 307-12
  • Peer Reviewed
• [Journal Article] The toxic effects and fate of intravenously administered zearalenone in goats. (2010)
  • Author(s): Dong M, He XJ, Tulayakul P, Li JY, Dong KS, Manabe N, Nakayama H, Kumagai S
  • Journal Title: Toxicon. 55(2-3) Pages: 523-30
  • Peer Reviewed
• [Journal Article] Determination of a yellow rice toxin, luteoskyrin, in rice by using liquid chromatography-tandem mass spectrometry with electrospray ionization. (2009)
  • Author(s): Mizutani K, Kumagai S, Mochizuki N, Kitagawa Y, Sugita-Konishi Y.
  • Journal Title: J Food Prot. 72(6) Pages: 1321-6
  • Peer Reviewed
• [Journal Article] Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. (2008)
  • Author(s): Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 25(9) Pages: 1101-6
  • Peer Reviewed
• [Journal Article] Detection of deoxynivalenol contamination in wheat products in Thailand. (2008)
  • Author(s): Poapolathep A, Poapolathep S, Klangkaew N, Sugita-Konishi Y, Kumagai S
  • Journal Title: J Food Prot. 71(9) Pages: 1931-3
  • Peer Reviewed
• [Journal Article] Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. (2008)
  • Author(s): Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y.
  • Journal Title: Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 25(9) Pages: 1101-6
  • Peer Reviewed