Study on prevalence of avian influenza viruses circulating among poultry reared in farms in northern Vietnam

2010 Fiscal Year Final Research Report

• Project/Area Number: 20405043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Nagasaki University
• Principal Investigator: YAMASHIRO Tetsu Nagasaki University, 熱帯医学研究所, 教授 (00244335)
• Co-Investigator(Kenkyū-buntansha): OOTSUKI Kouichi 京都産業大学, 先端科学技術研究所, 教授 (00032293) ; ITOU Toshihiro 鳥取大学, 農学部, 教授 (00176348)
• Project Period (FY): 2008 – 2010
• Keywords: ウイルス / 感染症 / 微生物 / 獣医学 / 鳥インフルエンザ / 家禽

Research Abstract

A total of 4,526 samples were collected from pigs, ducks and chickens, in winter seasons of 2009-2011, in farms in Hanoi. Of those, influenza A-specific M gene was detected in 52% (342/659) and in 29% (761/2,669), and 39% (468/1,198) the samples taken from pigs, ducks, and, chicken in the seasons, from which H6N1 and H9N2 subtypes of Avian influenza viruses were isolated. Phylogenetic analyses revealed that these two subtypes carried genes close to those isolated in South Korea, as well as in east southern China or in northern Japan, indicating that AIVs circulating among ducks in northern Vietnam were possibly introduced by wild birds migrating along with the East Asian flyway.

Research Products

• [Journal Article] Molecular epidemiology of avian influenza viruses circulating among he-althy poultry bred in farms in northern Vietnam. (2011)
  • Author(s): Takakuwa H., Yamashiro T., Le Q.M., Phuong LS, Ozaki H., Tsunekuni R., Usui T., Ito H., Morimatsu M., Yamaguchi T., Ito T.,Murase T., Ono E., Otsuki K.
  • Journal Title: Prev Vet Med. In press
• [Journal Article] Development of reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay for detection of avian influenza viruses in field specimens. (2010)
  • Author(s): Shivakoti S, Ito H, Murase T, Ono E, Takakuwa H, Yamashiro T, Otsuki K.
  • Journal Title: J.Vet.Med.Sci. 72 Pages: 513-524
• [Journal Article] Possible circulation of H5N1 avian influenza viruses in healthy ducks on farms in northern Vietnam. (2009)
  • Author(s): Takakuwa H, Yamashiro T, Le MQ, Phuong LS, Ozaki H, Tsunekuni R, Usui T, Ito H, Yamaguchi T, Ito T, Murase T, Ono E, Otsuki K.
  • Journal Title: Microbiol.Immunol. 54 Pages: 58-62
• [Presentation] Isolation and characterization of avian influenza viruses circulating among poultry reared in a village in Hanoi,2009 (2010)
  • Author(s): 堀田こずえ、高桑弘樹、村瀬敏之、小野悦郎、伊藤壽啓、大槻公一、山城哲
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2010
  • Place of Presentation: ベトナム メリアホテル
  • Year and Date: 2010-11-11
• [Presentation] ハノイ市郊外で飼育されるアヒルおよびブタより分離されたA型インフルエンザウイルスの遺伝子解析 (2010)
  • Author(s): 堀田こずえ、高桑弘樹、村瀬敏之、小野悦郎、伊藤壽啓、大槻公一、山城哲
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島 あわぎんホール
  • Year and Date: 2010-11-08