Analys is of exocytotic mechanism of BDNF from astrocytes.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kitasato University
• Principal Investigator: ITAKURA Makoto Kitasato University, 医学部, 講師 (30398581)
• Project Period (FY): 2008 – 2010
• Keywords: グリア細胞 / 開口放出 / 脳神経栄養因子 / プロテインキナーゼC / SNAP-23

Research Abstract

The brain are made up of many cells, including neurons and glial cells. Astrocytes, a type of glial cells release various bioactive substances via Ca(2+)-and SNARE-dependent exocytosis. In the present study, we revealed that potent protein kinase C activator, phorbol 12-myristate 13-acetate (PMA) treatment suppressed exogenously expressed human growth hormone release from cultured astrocytes. Moreover, PMA treatment induced the phosphorylation of synaptosomal-associated protein of 23 kDa on Ser(95), Ser(120), and Ser(160).

Research Products

• [Journal Article] M. PKC-dependent inhibition of CA2+-dependent exocytosis from astrocytes (2011)
  • Author(s): Yasuda, K., Itakura, M., Aoyagi, K., Sugaya, T., Nagata, E., Ihara, H., Takahashi
  • Journal Title: Glia 59 Pages: 143-151
  • Peer Reviewed
• [Presentation] M. Phorbol 12-myristate 13-acetate suppresses Cat+-dependent exocytotic release from rat astroglial cells (2009)
  • Author(s): Yasuda, K., Itakura, M., Aoyagi, K., Sugaya, T., Yamamori, S., Takahashi
  • Organizer: 第36回国際生理学会世界大会
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] M. Identification of PKC-dependent phosphorylation sites of SNAP-23inclonalglia cells (2009)
  • Author(s): Yasuda, K., Itakura, M., Nagata, E., Yamamori, S., Ihara, H., Takahashi
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-23