Functional analysis of GluD2 signal transduction

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500292
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: KOHDA Kazuhisa Keio University, 医学部, 講師 (40334388)
• Co-Investigator(Kenkyū-buntansha): EMI Kyoichi 慶應義塾大学, 医学部, 研究員(非常勤) (70468495)
• Project Period (FY): 2008 – 2010
• Keywords: デルタ2グルタミン酸受容体 / 長期抑圧 / 小脳 / シナプス可塑性

Research Abstract

We performed functional analysis of the δ2 glutamate receptor (δ2) through phenotype-rescue method. By transducing an N-terminal mutant of δ2 into δ2-null Purkinje cells, we demonstrated that the extracellular N-terminal region of δ2 was indispensable for parallel fiber (PF)-Purkinje cell (PC) synapse formation and/or maintenance, but not for long-term depression (LTD), a form of plasticity in PF-PC synapses. We also found that PTPMEG, a tyrosine phosphatase interacting with the C-terminal region of δ2, dephosphorylated type-2 glutamate receptors in the induction of LTD.

Research Products

• [Journal Article] D-Serine regulates cerebellar LTD and motor coordination through the delta2 glutamate receptor. (2011)
  • Author(s): Kakegawa W, Miyoshi Y, Hamase K, Matsuda S, Matsuda K, Kohda K, Emi K, Motohashi J, Konno R, Zaitsu K, Yuzaki M
  • Journal Title: Nat Neurosci 14 Pages: 603-6011
  • Peer Reviewed
• [Journal Article] The N-terminal domain of GluD2 (GluRδ2) recruits presynaptic terminals and regulates synaptogenesis in the cerebellum in vivo (2009)
  • Author(s): Kakegawa W, Miyazaki T, Kohda K, Matsuda K, Emi K, Motohashi J, Watanabe M, Yuzaki M
  • Journal Title: J Neurosci 29 Pages: 5738-48
  • Peer Reviewed
• [Journal Article] Differential regulation of synaptic plasticity and cerebellar motor learning by the C-terminal PDZ-binding motif of GluRdelta2. (2008)
  • Author(s): Kakegawa W, Miyazaki T, Emi K, Matsuda K, Kohda K, Motohashi J, Mishina M, Kawahara S, Watanabe M, Yuzaki M
  • Journal Title: J Neurosci 28 Pages: 1460-68
  • Peer Reviewed
• [Journal Article] Phosphorylation of delta2 glutamate receptors at serine 945 is not required for cerebellar long-term depression. (2008)
  • Author(s): Nakagami, R., Kohda, K., Kakegawa, W., Kondo, T., Kato, N., Yuzaki, M.
  • Journal Title: Keio J Med 57 Pages: 105-110
  • Peer Reviewed
• [Journal Article] A New Rapid Protocol for Eyeblink Conditioning to Assess Cerebellar Motor Learning.
  • Author(s): Emi K, Kohda K, Kakegawa W, Yuzaki M
  • Journal Title: Neurochemical Res 2011 (E-pub ahead of print)
  • Peer Reviewed
• [Presentation] 小脳長期抑制とTARPによる制御 (2010)
  • Author(s): 野村寿博、掛川渉、松田信爾、幸田和久、柚崎通介
  • Organizer: 第33回日本神経学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] 瞬目条件付け学習の消去と保持には平行線維-プルキンエ細胞シナプスは関与しない-Cbln1欠損マウスを用いた解析 (2009)
  • Author(s): 江見恭一、三浦会里子、掛川渉、幸田和久、渡辺雅彦、柚崎通介
  • Organizer: 第32回日本神経科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Cbln1欠損マウスにおける瞬目条件付け学習障害は成熟後のCbln1投与により急速に回復する (2008)
  • Author(s): 江見恭一、石田綾、近藤哲郎、幸田和久、柚崎通介
  • Organizer: 第31回日本神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] 内在性D-セリンはNADA 受容体とデルタ2受容体を介して小脳LTDを制御する (2008)
  • Author(s): 掛川渉、幸田和久、柚崎通介
  • Organizer: 第31回日本神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Remarks] ホームページ等