Analysis of the role of a protein tyrosine phosphatase in regulation of neuronal functions

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Gunma University
• Principal Investigator: OHNISHI Hiroshi Gunma University, 生体調節研究所, 准教授 (70334125)
• Project Period (FY): 2008 – 2010
• Keywords: チロシンリン酸化 / 脳 / ストレス

Research Abstract

To investigate the functional roles of cytoplasmic protein tyrosine phosphatase Shp2 in the brain, I have generated adult forebrain specific Shp2 conditional KO (cKO) mice by the use of Cre-LoxP system. The effect of Shp2 ablation on the brain functions is now being examined in the cKO mice by the use of several behavioral tests. In this project, I have also investigated a signaling mechanism of tyrosine phosphorylation of SIRPa, which is a major binding protein of Shp2 in the brain, and found that cold-stress induced tyrosine phosphorylation of SIRPa in the brain.

Research Products

• [Journal Article] Stress-evoked tyrosine phosphorylation of signal regulatory protein a regulates behavioral immobility in the forced swim test. (2010)
  • Author(s): Ohnishi H., MurataT., Kusakari S., Hayashi Y., Takao K., Maruyama T., Ago Y., Koda K., Jin F.-J., Okawa K., Oldenborg P.-A., Okazawa H., Murata Y., Furuya N., Matsuda M., Miyakawa T., Matozaki T.
  • Journal Title: J.Neurosci. Vol.30 Pages: 10472-10483
  • Peer Reviewed
• [Journal Article] Regulation by SIRPa of dendritic cell homeostasis in lymphoid tissues. (2010)
  • Author(s): Saito Y., Iwamura H., Kaneko T.,Ohnishi H., Murata Y., Okazawa, H., Kanazwa Y., Sato-Hashimoto M., Kobayashi H., Oldenborg P.-A., Naito M., Kaneko Y., Nojima Y., Matozaki T.
  • Journal Title: Blood. Vol.116 Pages: 3517-3525
  • Peer Reviewed
• [Journal Article] Functions and molecular mechanisms of the CD47-SIRPa signaling pathway. (2009)
  • Author(s): Matozaki T., Murata Y., Okazawa H., Ohnishi H.
  • Journal Title: Trends Cell Biol. Vol.19 Pages: 72-80
  • Peer Reviewed
• [Journal Article] Protein tyrosine phosphatase SHP-2 ; a proto-oncogene product that promotes Ras activation. (2009)
  • Author(s): Matozaki T., Murata Y., Mori M., Kotani T., Okazawa H., Ohnishi H.
  • Journal Title: Cancer Sci. Vol.100 Pages: 1786-1793
  • Peer Reviewed
• [Journal Article] Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47-SHPS-1 system. (2008)
  • Author(s): Kusakari S., Ohnishi H., Jin F.-J., Kaneko Y., Murata T., Murata Y., Okazawa H., Matozaki T.
  • Journal Title: J.Cell Sci. Vol.121 Pages: 1213-1223
  • Peer Reviewed
• [Presentation] Stress-evoked tyrosine phosphorylation of SIRPa mediates an antidepressant effect (2010)
  • Author(s): Ohnishi H., Kusakari S., Murata T., Hayashi Y., Maruyama T., Okazawa H., Murata Y., Takao K., Miyakawa T., Oldenborg P.-A., Matozaki T.
  • Organizer: 40th Annual Meeting of Society for Neuroscience, Neuroscience 2010
  • Place of Presentation: San Diego Convention Center(サンディエゴ、米国)
  • Year and Date: 2010-11-16
• [Presentation] チロシンリン酸化シグナルによる脳のストレス応答制御 (2010)
  • Author(s): 大西浩史、草苅伸也、村田考啓、丸山登士、林由里子、高雄啓三、宮川剛、吾郷由希夫、香田健、松田敏夫、大川克也、齊藤泰之、村田陽二、的崎尚
  • Organizer: Neuro 2010(第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会、合同大会)
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-09-02
• [Presentation] チロシンリン酸化シグナルによる脳のストレス応答制御 (2009)
  • Author(s): 大西浩史、草苅伸也、林由里子、高雄啓三、宮川剛、齊藤泰之、村田陽二、的崎尚
  • Organizer: 第52回日本神経化学会大会
  • Place of Presentation: ホテル天保(群馬県)
  • Year and Date: 2009-06-22
• [Presentation] CD47とSHPS-1のトランスエンドサイトーシスとCD47-SHPS-1系の制御における役割 (2008)
  • Author(s): 大西浩史、草苅伸也、村田考啓、村田陽二、岡澤秀樹、的崎尚
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2008-07-01
• [Remarks] ホームページ等
  • URL: http://www.imcr.gunma-u.ac.jp/lab/biosig/