2010 Fiscal Year Final Research Report
Identification of membrane targets for 15-deoxy Δ^<12,14>-prostaglandin J_2
| Project/Area Number |
20500346
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Himeji Dokkyo University |
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Principal Investigator |
YAGAMI Tatsurou Himeji Dokkyo University, 薬学部, 教授 (00363812)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASE Kenkichi 姫路獨協大学, 薬学部, 講師 (80381474)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 神経科学 / 薬理学 / 脳 / 脳神経疾患 / 脂質 |
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| Research Abstract |
We found that specific binding sites of 15-deoxy-Δ^<12,14>-prostaglandin J_2 (15d-PGJ_2) was involved in the neurodegeneration. A proteomic approach was used to identify protein targets for 15d-PGJ_2 in the plasma membrane. By using biotinylated 15d-PGJ_2, eleven proteins were identified as biotin-positive spots and classified into three different functional proteins : glycolytic enzymes (Enolase2, pyruvate kinase M1 (PKM1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH)), molecular chaperones (heat shock protein 8 and T-complex protein 1 subunit α), cytoskeletal proteins (Actin β, F-actin-capping protein, Tubulin β and Internexin α). GAPDH, PKM1 and Tubulin β are Aβ-interacting proteins.
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[Presentation]
Author(s)
Yagami T
Organizer
Biological Effects of Blood Glucose Level Control Pharmaceutical Sciences World Congress 20101114-20101118
Place of Presentation
New Orleans, Louisiana, USA(invited)
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