Mechanisms of endocannabinoid-mediated synaptic plasticity

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500357
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kanazawa University
• Principal Investigator: SHOSAKU Takako Kanazawa University, 保健学系, 教授 (60179025)
• Research Collaborator: ECHIGO Ryousuke 金沢大学, 大学院・医学系研究科保健学専攻, 博士後期課程2年
• Project Period (FY): 2008 – 2010
• Keywords: ニューロン・シナプス・神経回路 / シナプス可塑性

Research Abstract

In the present study, we investigated the conditions inducing long-lasting endocannabinoid release, which is required for endocannabinoid-mediated long-term depression. We demonstrated that protease-activated receptor-1 (PAR1), which can be activated persistently by proteases, can induce endocannabinoid release when activated. We observed that the activation of group 1 metabotropic glutamate receptors or M_1/M_3 muscarinic receptors occasionally induced long-lasting endocannabinid release, which could not be attributed to long-lasting enhancement of PLCβ.

Research Products

• [Journal Article] Neuronal protease-activated receptor 1 drives synaptic retrograde signaling mediated by the endocannabinoid 2-arachidonoylglycerol (2011)
  • Author(s): Hashimotodani, Y., Ohno-Shosaku, T., Yamazaki, M., Sakimura, K., Kano, M.
  • Journal Title: J.Neurosci. 31 Pages: 3104-3109
  • Peer Reviewed
• [Journal Article] Endocannabinoid-mediated control of synaptic transmission (2009)
  • Author(s): Kano M., Ohno-Shosaku T., Hashimotodani Y., Uchigashima M., Watanabe M.
  • Journal Title: Physiol.Rev. 89 Pages: 309-380
  • Peer Reviewed
• [Journal Article] Endocannabinoids and retrograde modulation of synaptic transmission
  • Author(s): Ohno-Shosaku, T., Tanimura, A., Hashimotodani, Y., Kano, M.
  • Journal Title: Neuroscientist in press
  • Peer Reviewed
• [Presentation] 向精神薬クロザピンの活性代謝物N-desmethylclozapineの海馬興奮性ニューロンに対するM1アゴニスト作用 (2011)
  • Author(s): 少作隆子, 長澤圭祐, 村西千穂, 菅原優翔, 米田貢
  • Organizer: 第88回日本生理学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)(震災のため誌上開催となり大会は中止)
  • Year and Date: 2011-03-28
• [Presentation] Modulation of hippocampal inhibitory synaptic transmission through protease-activated receptor 1 (2009)
  • Author(s): Hashimotodani, Y., Ohno-Shosaku T., Kano, M.
  • Organizer: 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center (Kyoto)
  • Year and Date: 2009-07-28
• [Presentation] Effects of intracellular Ca^<2+> level on channel modulation induced by activation of muscarinic receptors in rat cultured hippocampal neurons (2009)
  • Author(s): Ohno-Shosaku T., Echigo, R., Kashima, K., Minami, H., Muranishi M., Nishikawa, Y., Taguchi, H., Watanabe, M.
  • Organizer: 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto International Conference Center (Kyoto)
  • Year and Date: 2009-07-28