2010 Fiscal Year Final Research Report
Development of the analysis system for the function of glycosylation-related genes involved in embryonic lethality
| Project/Area Number |
20500376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagoya City University |
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Principal Investigator |
MIYOSHI Ichiro Nagoya City University, 大学院・医学研究科, 教授 (10183972)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 糖鎖 / 遺伝子 / 発生分化 / 実験動物学 / セラミド細胞内選択輸送タンパク質 |
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| Research Abstract |
We attempted to establish the analysis system for the function of Ugcg(UDP-Glucose Ceramide Glucosyltransferase) gene on the basis of the quantitative difference, from complete deficiency to overexpression, in the expression of homologue gene introduced into Ugcg-deficient ES cell on both alleles. However, stably transfected clone was note obtained. We generated Ceramide Transfer Protein (Cert) conditional knockout mice deficient in exon5 by using the Cre-LoxP system. As Cert proved to be an essential gene for mouse development and embryonic survival, this mice is a new model useful for the study of the function of glycosylation-related genes involved in embryonic lethality.
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Research Products
(9 results)
