2010 Fiscal Year Final Research Report
Basic study on follicular oocyte maturation by in vitro culture and production of offspring from embryos derived from IVM-maturation oocytes
| Project/Area Number |
20500379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nihon University |
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Principal Investigator |
SATO Kahei Nihon University, 生物資源科学部, 教授 (20059778)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Naoto 日本大学, 生物資源科学部, 講師 (70297795)
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Nao 聖マリアンナ医科大学, 医学部, 講師 (90246356)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 卵胞卵子成熟 / 共培養 / リコンビナントFSH / IVM / vitrification |
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| Research Abstract |
In this study, using recombinant FSH (recFSH) the in vitro maturation (IVM) of mouse follicular oocytes was investigated. Using recFSH IVM showed high maturation rate of the oocytes obtained from fresh or frozen/thawed ovarian tissues. Fertilization of the mature oocytes obtained showed high rate and its embryos developed into offspring by transfer into foster mothers. Co-culture of follicular oocytes with mouse fibroblasts also resulted in constant maturation rates compared with control (in vivo maturation oocytes).
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Research Products
(5 results)
