2010 Fiscal Year Final Research Report
Specific and quantitative detection of LAMP products from pathogens of laboratory animals using a newly developed electrochemical DNA chip.
| Project/Area Number |
20500381
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Teikyo University (2009-2010)
Central Institute for Experimental Animals (2008) |
|---|
Principal Investigator |
GOTO Kazuo Teikyo University, 医療技術学部, 准教授 (00205593)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HAYASHIMOTO Nobuhito 実験動物中央研究所, 実験動物研究部, 研究員 (30332208)
YASUDA Masahiko 実験動物中央研究所, 実験動物研究部, 研究員 (40353479)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | ヘリコバクター / DNAチップ / LAMP法 / PCR法 |
|---|
| Research Abstract |
We developed a microfabricated electrochemical DNA chip for detection of LAMP/PCR products from 16S rRNA sequences of Helicobacter spp. This chip does not require DNA labeling, and the hybridization signal can be detected as an anodic current. The average anodic currents of 5 (H.bilis) and 8 (H.hepaticus) PCR positive samples derived from feces of spontaneously infected mice (Cp, Hb and Hh) were 27.9±7.2, 31.9±8.1, 29.3±10.1, and 27.6±3.0 nA, respectively. On the other hand, the average anodic currents of 27 (Hb) and 18 (Hh), PCR negative samples were 3.7±2.4 and - 1.0±1.7, and nA, respectively. The anodic current increased with increasing concentrations of pathogens. For experimentally infected samples, the results of PCR/electrophoresis were in complete accord with those of this system when anodic currents of 8.5 (Hb) and 2.4 (Hh) nA were taken as the cut-off value. The results suggested that the electrochemical DNA chip system is useful for specific and quantitative detection of LAMP/PCR products.
|
