2010 Fiscal Year Final Research Report
A high-speed congenic strategy using first-wave male germ cells
| Project/Area Number |
20500383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
OGONUKI Narumi The Institute of Physical and Chemical Research, 遺伝工学基盤技術室, 専任技師 (40373287)
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| Project Period (FY) |
2008 – 2010
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| Keywords | マウス / 実験動物 / 発生工学 / 顕微授精 |
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| Research Abstract |
To establish high-speed congenic system, microinsemination with round spermatid which retrieved from immature mouse selected by marker-assisted screening system was tried. Nine congenic strains were produced by this method for 106 and 190 days of N3 to N5 generation. The generation turnover time in mice can be shorted to about 40 days by using the first wave round spermatids as male gametes. This method can establish congenic strain the period below about half of conventional method.
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[Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.2010
Author(s)
Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A
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Journal Title
Science 330
Pages: 496-499
Peer Reviewed
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[Journal Article] A high-speed congenic strategy using first-wave male germ cells.2009
Author(s)
Ogonuki N, Inoue K, Hirose M, Miura I, Mochida K, Sato T, Mise N, Mekada K, Yoshiki A, Abe K, Kurihara H, Wakana S, Ogura A
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Journal Title
PLoS One 4(3)
Pages: e4943
Peer Reviewed
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[Journal Article] Efficient production of androgenetic embryos by round spermatid injection.2009
Author(s)
Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A
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Journal Title
Peer Reviewed
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[Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries.2009
Author(s)
Honda A, Hirose M, Inoue K, Hiura H, Miki H, Ogonuki N, Sugimoto M, Abe K, Kanatsu-Shinohara M, Kono T, Shinohara T, Ogura A
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Journal Title
Int J Dev Biol 53
Pages: 605-613
Peer Reviewed
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[Journal Article] Stable ES cell lines in rabbits-potential small animal models for human ES cell research.2008
Author(s)
Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N, Hatori M, Shimizu N, Murata T, Hirose H, Katayama K, Wakisaka N, Miyoshi H, Yokoyama KK, Sankai T, Ogura A
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Journal Title
Reprod Biomed Online 17
Pages: 706-715
Peer Reviewed
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