2010 Fiscal Year Final Research Report
Cardiac action potential and Ca2+ ion simultaneous dual measurement and temporal and spatial high resolution optical mapping system
| Project/Area Number |
20500387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SAKUMA Ichiro The University of Tokyo, 大学院・工学系研究科, 教授 (50178597)
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| Co-Investigator(Renkei-kenkyūsha) |
KODAMA Itsuo 名古屋大学, 環境医学研究所, 教授 (30124720)
HONJO Haruo 名古屋大学, 環境医学研究所, 准教授 (70262912)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 生体情報 / 計測 |
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| Research Abstract |
We developed optical mapping system which records two signals simultaneously using single camera by taking each frame synchronized with filter switching. We designed the optical lens system to concentrate fluorescent light at filter position and form image on the CMOS sensor, additionally a custom filter holder for long-pass filter and band-pass filter which is rotated by a motor. Using this system, we observed Vm and Ca2+ dynamics in isolated rabbit heart. Firstly in the first step of basic study, there ware many difficult about the optical design of measuring system. Then we developed another solution for exquisite Vm and Ca dual mapping system that was constructed by 2 camera, dichroic mirror and Mutual Information based registration software both.
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