PEG-grafted linear cationic polymer for tumor accumulation and cell penetration

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500410
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyushu University
• Principal Investigator: KANO Arihiro Kyushu University, 先導物質化学研究所, 准教授 (30403950)
• Co-Investigator(Renkei-kenkyūsha): MARUYAMA Atsushi 九州大学, 先導物質化学研究所, 教授 (40190566)
• Project Period (FY): 2008 – 2010
• Keywords: 薬物伝達システム / 核酸医薬 / カチオン性高分子 / 腫瘍集積性

Research Abstract

We previously showed that polyethyleneglycol-grafted poly-L-lysine (PLL-g-PEG) exerts long lifetime of nucleic acids medicine, such as siRNA, in blood circulation. It is conceivable that PLL-g-PEG is useful as a drug delivery carrier for tumor treatment. In this study, we examined the effects of PEG grafting ratio and the length of the PEG chain and PLL backbone on the stability of siRNA/PLL-g-PEG complex and the tumor accumulation. As the results, it was revealed that the higher PEG-grafted PLL, more than 30%, is efficiently accumulated in tumor tissues and even the affinity to the siRNA is augmented.

Research Products

• [Journal Article] Grafting of poly (ethylene glycol) to poly-lysine augments its lifetime in blood circulation and accumulation in tumors without loss of the ability to associate with siRNA. (2011)
  • Author(s): Kano, A., Moriyama, K., Yamano, T., Nakamura, I., Shimada, N., Maruyama, A.
  • Journal Title: J Control Release 149(1) Pages: 2-7
  • Peer Reviewed
• [Journal Article] Polymer brush-Stabilized polyplex for a siRNA carrier with long blood circulatory half-life. Adv. Mater. Res. (2008)
  • Author(s): Kano, A., Yamano, T., Choi, S.W., Maruyama, A.
  • Journal Title: Adv. Mater. Res. (Zuerich, Switz.) FIELD Full Journal Title : Advanced Materials Research (Zuerich, Switzerland)(Pt.1, Multi-Functional Materials and Structures) Pages: 47-50,762-764
  • Peer Reviewed
• [Presentation] Complex Formation and Tumor Accumulation of Porfimer Sodium with PLL-g-PEG Copolymer for Photodynamic Therapy (2010)
  • Author(s): Arihiro Kano, Yuuki Taniwaki, Takeshi Yamano, Izumi Nakamura, Naohiko Shimada, Atsushi Maruyama
  • Organizer: 2010 International Symposium of Materials on Regenerative Medicine
  • Place of Presentation: Zhunan, Taiwan
  • Year and Date: 2010-11-05
• [Presentation] 水溶性グラフト高分子の腫瘍集積性とドラッグデリバリー (2010)
  • Author(s): 狩野有宏
  • Organizer: 九州地区高分子若手研究会・夏の講演会
  • Place of Presentation: 北九州
  • Year and Date: 2010-07-09
• [Presentation] PLL-g-PEG共重合体の腫瘍集積性の解析とsiRNAデリバリーへの応用 (2008)
  • Author(s): 狩野有宏、山野剛、丸山厚
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-29
• [Remarks] ホームページ等
  • URL: http://www.cm.kyushu-u.ac.jp/wmaruyama/