低エネルギー超音波を利用した新しい非侵襲的癌治療システムの確立

2008 Fiscal Year Annual Research Report

• Project/Area Number: 20500432
• Research Institution: Fukuoka University
• Principal Investigator: LORETO B Feril Fukuoka University, 医学部, 講師 (10435109)
• Co-Investigator(Kenkyū-buntansha): 立花 克郎 福岡大学, 医学部, 教授 (40271605) ; 遠藤 日富美 福岡大学, 医学部, 助教 (00435108)
• Keywords: low-intensity / Bioeffects / Sonotransfection / Cancer Therapy / Sonoporation

Research Abstract

2008年からの研究で、我々は、in vitro、in vivo、双方で低エネルギー超音波照射によりメラノーマ細胞(C32)の増殖を抑制することを確認した。この効果は子宮頸癌細胞(HeLa)にもある程度認められた。しかし、良性のメラノサイトや使用した他の癌細胞(U937,MCF-7, MDA)では増殖抑制効果は見られなかった。ジーンチップを用いた予備実験で65,000の遺伝子を解析し、900以上の遺伝子変異を確認した。一方、in vitroの実験系での細胞増殖抑制率のばらつきが認められ、超音波強度の設定条件を決めることが困難であった。低エネルギー超音波に対する生体反応に一定の閾値があるものと予想された。超音波強度0.5W/cm 2以下で、全く細胞増殖抑制効果が認められない細胞種が認められ、今後は再実験を行うことで上記の現象をさらに確認していく必要がある。
本年度は低エネルギー超音波照射による遺伝子変異に焦点をしぼり研究を行い、細胞増殖抑制のメカニズムを解明したい。
癌細胞をアポトーシスに導く超音波照射の最適条件の研究は、細胞を低張状態で膨張させて超音波を照射し、その相乗効果を検討する。超音波と低張状態の併用は、細胞膜をより危弱にすることで相乗効果が期待できる。

Research Products

• [Journal Article] Evaluation and comparison of three novel microbubbles : Enhancement of ultrasound-induced cell death and free radicals production. (2009)
  • Author(s): Hassan MA
  • Journal Title: Ultrasonics Sonochemistry 16 Pages: 372-378
  • Peer Reviewed
• [Journal Article] Influence of Changing Pulse Repetition Frequency on Chemical and Biological Effects Induced by Low Intensity Ultrasound In-vitro. (2009)
  • Author(s): Buldakov MA
  • Journal Title: Ultrasonics Sonochemistry 16 Pages: 392-397
  • Peer Reviewed
• [Journal Article] Construction of artificial promoters sensitively responsive to sonication in vitro. (2009)
  • Author(s): Watanabe A
  • Journal Title: Journal of Medical Ultrasonics 36 Pages: 9-17
  • Peer Reviewed
• [Journal Article] Growth inhibition of neurofibroma by ultrasound-mediated interferon gamma transfection (2009)
  • Author(s): Yamaguchi K
  • Journal Title: Journal of Medical Ultrasonics 36 Pages: 3-8
  • Peer Reviewed
• [Journal Article] Gene Transfection through microbubble-aided sonication in cancer cells (2009)
  • Author(s): Dickerson C. Moreno
  • Journal Title: Philippine Physics Journal 31 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Combination of doxorubicin and low-intensity ultrasound causes a synergistic enhancement in cell killing and an additive enhancement in apoptosis induction in human lymphoma U937 cells. (2008)
  • Author(s): Yoshida T
  • Journal Title: Cancer Chemotherapy and Pharmacology 61 Pages: 559-567
  • Peer Reviewed
• [Journal Article] Effects of ultrasound on apoptosis induced by anti-CD20 antibody in CD20-positive B lymphoma cells. (2008)
  • Author(s): Danno D
  • Journal Title: Ultrasonics Sonochemistry 15 Pages: 463-471
  • Peer Reviewed
• [Journal Article] Sonodynamic therapy (2008)
  • Author(s): Tachibana K
  • Journal Title: Ultrasonics 48 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells. (2008)
  • Author(s): Tabuchi Y
  • Journal Title: Cancer Letters 270 Pages: 286-294
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-intensity ultrasound (part 1) : thermal and sonodynamic effects. (2008)
  • Author(s): Feril LB Jr
  • Journal Title: Journal of Medical Ultrasonics 35 Pages: 153-160
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-intensity ultrasound (part 2) : biomolecular effects, sonotransfection, and sonoporation. (2008)
  • Author(s): Feril LB Jr
  • Journal Title: Journal of Medical Ultrasonics 35 Pages: 161-167
  • Peer Reviewed
• [Journal Article] Dual role of low-dose ionizing radiation. (2008)
  • Author(s): Buldakov AM
  • Journal Title: Philippine Physics Journal 30 Pages: 1-6
  • Peer Reviewed
• [Presentation] Updates on Therapeutic Ultrasound (2009)
  • Author(s): Feril LB Jr
  • Organizer: 7^<th> Annual Convention of the Philippine Society of Ultrasound in Clinical Medicine, Inc. (PSUCMI)
  • Place of Presentation: Quezon City Sports Club, Philippines
  • Year and Date: 20090320-20090322
• [Presentation] Effects of Ultrasound : Its Therapeutic Potential (2008)
  • Author(s): Feril LB Jr
  • Organizer: Institute of Biology, College of Science, University of the Philippines special meeting
  • Place of Presentation: Diliman, Quezon City, Philippines
  • Year and Date: 2008-11-24
• [Presentation] Low-intensity ultrasound inhibits cancer cell growth in vitro and tumor growth in vivo. (2008)
  • Author(s): Feril LB Jr., Tachibana K, Yamaguchi K, Ikeda-Dantsuji Y, Kondo T, Tabuchi Y, Furusawa Takasaki I.
  • Organizer: 8^<th> International Symposium on Therapeutic Ultrasound.
  • Place of Presentation: Minneapolis, MN, USA
  • Year and Date: 2008-09-12
• [Presentation] Basics of Diagnostic and Therapeutic Ultrasound (2008)
  • Author(s): Feril LB Jr
  • Organizer: Physics Department, College of Sciences, De La Salle University special meeting
  • Place of Presentation: Manila, Philippines
  • Year and Date: 2008-06-18
• [Presentation] Low-intensity ultrasound inhibits melanoma growth in vitro and in vivo. (2008)
  • Author(s): Feril LB Jr, Tachibana K, Yamaguchi K
  • Organizer: 20^<th> International Pigment Cell Conference and 5^<th> International Melanoma Research Congress.
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-11
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUnivHtml/info/4237/R110E.html