LOW-INTENSITY ULTRASOUND FOR CANCER THERAPY

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Fukuoka University
• Principal Investigator: LORETO B.Feril Fukuoka University, 医学部, 講師 (10435109)
• Co-Investigator(Kenkyū-buntansha): TACHIBANA Katsuro 福岡大学, 医学部, 教授 (40271605) ; ENDO Hitomi 福岡大学, 医学部, 講師 (00435108)
• Project Period (FY): 2008 – 2010
• Keywords: Ultrasound / Sonotransfection / Cancer Therapy / Sonoporation / Bioeffect

Research Abstract

Overall, this 3-year project underscores three major outcomes that may have significant impact contributing to the eventual use of low-intensity ultrasound for therapy, particularly cancer therapy. One is the successful sonotransfection resulting in growth inhibition of cancer cells in vitro and tumor growth in vivo. Another is sonodynamic therapy resulting in growth inhibition of cancer cells in vitro and tumor growth in vivo. In combination with antibiotic, sonodynamic therapy also enhanced antimicrobial effects. The other is the use of prolonged and repetitive exposure to low-intensity ultrasound, which resulted in inhibition of melanoma growth.

Research Products

• [Journal Article] Synergistic inhibition of malignant melanoma proliferation by melphalan combined with ultrasound and microbubbles. (2011)
  • Author(s): Matsuo M, Yamaguchi K, Feril LB Jr, Endo H, Ogawa K, Tachibana K, Nakayama J.
  • Journal Title: Ultrasonics Sonochemistry 18 Pages: 1218-1224
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Ultrasound and Antibiotics against Chlamydia trachomatis-infected Human Epithelial cells in vitro. (2011)
  • Author(s): Ikeda-Dantsuji Y, Feril LB Jr, Tachibana K, Ogawa K, Endo H, Harada Y, Suzuki R, Maruyama K.
  • Journal Title: Ultrasonics Sonochemistry 18(1) Pages: 425-430
  • Peer Reviewed
• [Journal Article] Ultrasound activation of TiO(2) in melanoma tumors. (2011)
  • Author(s): Harada Y, Ogawa K, Endo H, Feril LB Jr, Uemura T, Tachibana K.
  • Journal Title: J Control Release 149(2) Pages: 190-195
  • Peer Reviewed
• [Journal Article] Differential Cytotoxicity and sonosensitization by sanazole : Effect of cell type and acoustic parameters. (2011)
  • Author(s): Hassan MA, Furusawa Y, Zhao Q-L, Takasaki I, Feril LB Jr, Tachibana K, Kudo N, Minemura M, Sugiyama T, Kondo T.
  • Journal Title: J Med.Ultrasonics 38(2) Pages: 65-72
  • Peer Reviewed
• [Journal Article] Hypotonia-induced cell swelling enhances ultrasound-induced mechanical damage on cancer cells. (2010)
  • Author(s): Feril LB Jr, Tachibana K, Kondo T, Ogawa R, Zhao QL, Yamaguchi Ogawa K, Endo H, Irie Y, Harada Y.
  • Journal Title: J.Med.Ultrasonics 37 Pages: 3-8
  • Peer Reviewed
• [Journal Article] Therapeutic high intensity focused ultrasound. (2010)
  • Author(s): Feril LB Jr., Tachibana K, Kondo T.
  • Journal Title: Philippine Physics J. 32 Pages: 47-52
  • Peer Reviewed
• [Journal Article] The sonochemical and biological effects of three clinically-used contrast agents. (2010)
  • Author(s): Hassan MA, Feril LB Jr, Kudo N, Tachibana K, Kondo T, Riesz P.
  • Journal Title: Japanese Journal of Applied Physics 49 Pages: 07HF23(online)
  • Peer Reviewed
• [Journal Article] Growth inhibition of neurofibroma by ultrasound-mediated interferon gamma transfection. (2009)
  • Author(s): Yamaguchi K, Feril LB Jr, Harada Y, Endo H, Irie Y, Nakayama J, Tachibana K.
  • Journal Title: J.Medical Ultrasonics 36 Pages: 3-8
  • Peer Reviewed
• [Journal Article] Influence of Changing Pulse Repetition Frequency on Chemical and Biological Effects Induced by Low Intensity Ultrasound In-vitro. (2009)
  • Author(s): Buldakov MA, Hassan MA, Zhao Q-L, Feril LB Jr, Kudo N, Kondo T, Litvyakov NV, Bolshakov MA, Rostov VV, Cherdyntseva NV
  • Journal Title: Riesz P.Ultrasonics Sonochemistry 16 Pages: 392-7
  • Peer Reviewed
• [Journal Article] Low-intensity ultrasound adjuvant therapy : The enhancement of doxorubicin-induced cytotoxicity and the acoustic mechanisms involved. (2009)
  • Author(s): Kondo T, Yoshida T, Ogawa R, Hasaan MA, Furusawa Y, Zhao QL, Watanabe A, Morii A, Feril LB Jr, Tachibana K, Kitagawa H, Tabuchi Y, Takasaki I, Shehata MH, Kudo N, Tsukada K.
  • Journal Title: J.Med.Ultrasonics 36 Pages: 61-68
  • Peer Reviewed
• [Journal Article] Gene transfection through microbubble-aided sonication in cancer cells. (2009)
  • Author(s): Moreno DC, Feril LB Jr, Tachibana K, Irie Y.
  • Journal Title: Philippine Physics J. 31 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Jr (2009)
  • Author(s): Ogawa R, Lee S, Izumi H, Kagiya G, Yoshida T, Watanabe A, Morii A, Kakutani S, Kondo T, Feril LB Enhancement of artificial promoter activity by ultrasound-induced oxidative stress.
  • Journal Title: Utrason Sonochem 16 Pages: 379-386
  • Peer Reviewed
• [Journal Article] Evaluation and comparison of three novel microbubbles : Enhancement of ultrasound-induced cell death and free radicals production. (2009)
  • Author(s): Hassan MA, Feril LB Jr, Suzuki K, Kudo N, Tachibana K, Kondo T.
  • Journal Title: Ultrasonics Sonochemistry 16 Pages: 372-8
  • Peer Reviewed
• [Journal Article] Sonodynamic therapy. (2008)
  • Author(s): Tachibana K, FeFeril LB Jr, Ikeda-Dantsuji Y.
  • Journal Title: Ultrasonics 48 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Cancer Letters (2008)
  • Author(s): Tabuchi Y, Takasaki I, Zhao Q-L, Wada S, Hori T, Feril LB Jr, Tachibana K, Nomura Kondo T.
  • Journal Title: Genetic networks responsive to low-intensity pulsed ultrasound in human lymphoma U937 cells. 270(2) Pages: 286-94
  • Peer Reviewed
• [Journal Article] Dual role of low-dose ionizing radiation. (2008)
  • Author(s): Buldakov AM, Feril LB Jr.
  • Journal Title: Philippine Physics J. 30 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-intensity ultrasound (part 1) : thermal and sonodynamic effects. (2008)
  • Author(s): Feril LB Jr, Tachibana K, Ogawa K, Yamaguchi K, Solano I, Irie Y.
  • Journal Title: J.Medical Ultrasonics 35 Pages: 153-60
  • Peer Reviewed
• [Journal Article] J.Medical Ultrasonics (2008)
  • Author(s): Feril LB Jr, Tachibana K, Ikeda-Dantsuji Y, Endo H, Harada Y, Kondo T, Ogawa R.
  • Journal Title: Therapeutic potential of low-intensity ultrasound (part 2) : biomolecular effects, sonotransfection, and sonoporation. 35 Pages: 161-67
  • Peer Reviewed
• [Presentation] Inhibition of melanoma growth when subcultured with melanocytes sonotransfected interferon-beta gene. (2011)
  • Author(s): Feril LB Jr., Tachibana K, Yamaguchi K
  • Organizer: Sonoporation research meeting.
  • Place of Presentation: Fukuoka University
  • Year and Date: 2011-03-05
• [Presentation] Biological effects of ultrasound : Potential therapeutic applications and their implications on the safety of diagnostic ultrasound. (2011)
  • Author(s): Feril LB Jr, Tachibana K.
  • Organizer: 6^<th> Asian-Pacific Organization for Cell Biology (APOCB) Congress.
  • Place of Presentation: EDSA Shangri-La, Manila, Philippines
  • Year and Date: 2011-02-28
• [Presentation] Low intensity ultrasound inhibits melanoma cell growth in vitro and tumor growth in vivo. (2010)
  • Author(s): Feril LB Jr., Tachibana K, Yamaguchi K, Ikeda-Dantsuji Y, Kondo T, Tabuchi Y, Furusawa Takasaki I.
  • Organizer: 10^<th> International Symposium on Therapeutic Ultrasound
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-06-11
• [Presentation] HIFU : Updates on Therapeutic Ultrasound (2010)
  • Author(s): Feril LB Jr.U, Tachibana K.
  • Organizer: 8^<th> Annual Convention of the Philippine Society of Ultrasound in Clinical Medicine, Inc. (PSUCMI).
  • Place of Presentation: Diamond Hotel, Manila, Philippines
  • Year and Date: 2010-03-19
• [Presentation] Medical education and public health in the Philippines. (2010)
  • Author(s): Feril LB Jr.
  • Organizer: Fukuoka Philippine Friendship Association
  • Place of Presentation: Fukuoka City, Japan
  • Year and Date: 2010-01-11
• [Presentation] Echo-contrast agent, Sonazoid, enhances inhibition of cancer cell growth by ultrasound in vitro, but not in vivo. (2009)
  • Author(s): Feril LB Jr, Tachibana K, Yamaguchi K, Ikeda-Dantsuji Y, Kondo T, Tabuchi Y, Furusawa Y, Takasaki I, Matsuo M.
  • Organizer: 11^<th> International Symposium on Ultrasound Contrast Imaging ; and 1^<st> ASIAN Conference on Ultrasound Contrast Imaging
  • Place of Presentation: Kunming, China
  • Year and Date: 2009-12-19
• [Presentation] Hypotonia-induced cell swelling enhances ultrasound-induced mechanical damage on cancer cells. (2009)
  • Author(s): Feril LB Jr, Tachibana K, Kondo T, Ogawa R, Zhao QL, Yamaguchi Ogawa K, Endo H, Irie Y, Harada Y.
  • Organizer: 12^<th> Congress of the World Federation for Ultrasound in Medicine and Biology.
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2009-09-01
• [Presentation] Updates on Therapeutic Ultrasound (2009)
  • Author(s): Feril LB Jr, Tachibana K
  • Organizer: 7^<th> Annual Convention of the Philippine Society of Ultrasound in Clinical Medicine, Inc. (PSUCMI).
  • Place of Presentation: Quezon City Sports Club, Philippines
  • Year and Date: 2009-03-21
• [Presentation] Dreaming the Impossible Dreams (2009)
  • Author(s): Feril LB Jr.
  • Organizer: Saint Theresa College
  • Place of Presentation: Tandag, Surigao del Sur.
  • Year and Date: 2009-03-15
• [Presentation] Effects of Ultrasound : Its Therapeutic Potential (2008)
  • Author(s): Feril LB Jr., Tachibana K.
  • Organizer: Institute of Biology, College of Science
  • Place of Presentation: University of the Philippines, Diliman, Quezon City, Philippines
  • Year and Date: 2008-11-24
• [Presentation] Low-intensity ultrasound inhibits cancer cell growth in vitro and tumor growth in vivo. (2008)
  • Author(s): Feril LB Jr., Tachibana K, Yamaguchi K, Ikeda-Dantsuji Y, Kondo T, Tabuchi Y, Furusawa Takasaki I.
  • Organizer: 8^<th> International Symposium on Therapeutic Ultrasound.
  • Place of Presentation: Minneapolis, MN, USA
  • Year and Date: 2008-09-12
• [Presentation] Basics of Diagnostic and Therapeutic Ultrasound (2008)
  • Author(s): Feril LB Jr., Tachibana K.
  • Organizer: College of Sciences, De La Salle University-Manila, Philippines
  • Year and Date: 2008-06-18
• [Presentation] Low-intensity ultrasound inhibits melanoma growth in vitro and in vivo. (2008)
  • Author(s): Feril LB Jr, Tachibana K, Yamaguchi K
  • Organizer: 20^<th> International Pigment Cell Conference and 5^<th> International Melanoma Research Congress.
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2008-05-11
• [Book] Sonodynamic therapy.(Therapeutic ultrasound : mechanisms to applications.) (2011)
  • Author(s): Feril LB Jr, Tachibana K, Kondo T, Campbell PA.
  • Total Pages: 279-295
  • Publisher: Nova Publishing, Hauppauge NY, USA.
• [Book] Gene Therapy of Cancer [Methods in Molecular Biology 2nd Edition, 2009] Ultrasound-mediated gene transfection
  • Author(s): Feril LB Jr.
  • Total Pages: 179-194
  • Publisher: eril Humana Press, Hatfield, Hertfordshire, AL10 9AB, UK.