2010 Fiscal Year Final Research Report
Patho-mechanism of transmission of low back pain
| Project/Area Number |
20500439
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Chiba University |
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Principal Investigator |
OHTORI Seiji Chiba University, 大学院・医学研究院, 助教 (40361430)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kazuhisa 千葉大学, 大学院・医学研究院, 教授 (20179477)
TAKASO Masashi 北里大学, 医学部附属病院, 教授 (90439117)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 椎間板 / 腰痛 / 細胞内シグナル / サイトカイン / グリア |
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| Research Abstract |
Study Design : Immunohistological analysis in an injured intervertebral disk (IVD) model.Objective : To elucidate and compare in rats the behavior of the sensory nervous system and inflammatory mediators in experimentally-injured IVDs.Summary of Background Data : Multiple human and animal studies have verified the presence of sensory nerve fibers in intervertebral disks or investigated the behavior of inflammatory mediators in injured IVDs, but no in vivo study to date has examined the relationship between the two.Methods : Eight-week-old female rats were used. In the disk-injured group, L5/6 disks were injured with a 24-gauge needle ; simultaneously, the neurotracer Fluoro-gold (FG) was injected into the L5/6 IVD. The L5/6 IVD, dorsal root ganglia (DRGs) from the L1 to L6 levels, and the spinal cord were resected at several time points after surgery. Nerve growth factor (NGF), tumor necrosis factor (TNF)-alpha and interrleukin (IL)-6 production in the IVDs were quantified using enzyme
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-linked immunosorbent assay (ELISA). DRGs were immunostained for calcitonin gene-related peptide (CGRP), and spinal cord sections were immunostained for ionized calcium-binding adaptor molecule-1 (iba1) and glial fibrillary acidic protein (GFAP).Results : NGF, and TNF-alpha levels (through 1 week) and IL-6 levels (through 4 days) were significantly higher in the disk-injured group than in the non-injured group (p<0.05). However, starting at 2 weeks (NGF and TNF-alpha) or 1 week (IL-6), the differences in inflammatory mediator levels between the two groups no longer were significant. In contrast, the percentage of CGRP-immunoreactive (IR) neurons among FG-labeled DRG neurons, and the numbers of iba1-IR microglia and GFAP-IR astrocytes in the spinal dorsal horn remained significantly higher in the injured group than in the non-injured group at all time points (p<0.05).Conclusion : Disk injury in rats produces persistent increases in neuropeptides in DRGs and glia in the spinal cord, but only transient increases in inflammatory mediators in IVDs. Less
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[Journal Article] Less invasive and less technically demanding decompressive procedure for lumbar spinal stenosis-appropriate for general orthopaedic surgeons?2011
Author(s)
Takaso M, Nakazawa T, Imura T, Okada T, Fukushima K, Ueno M, Saito W, Shintani R, Sakagami H, Takahashi K, Yamazaki M, Ohtori S, Kotani T
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Journal Title
Int Orthop. 35
Pages: 67-73
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[Journal Article] Effect of applying p75NTR saporin to a punctured intervertebral disc on calcitonin gene-related peptide expression in rat dorsal root ganglion neurons.2010
Author(s)
Sugiura A, Ohtori S, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Norimoto M, Orita S, Eguchi Y, Kuniyoshi K, Ochiai N, Kishida S, Takaso M, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Takahashi K
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Journal Title
J Orthop Sci. 15
Pages: 407-13
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[Journal Article] Primary sensory neurons with dichotomizing axons projecting to the facet joint and the low back muscle in rats.2010
Author(s)
Wakai K, Ohtori S, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Fukui Y, Hayashi Y, Aoki Y, Kuniyoshi K, Nakamura J, Ishikawa T, Arai G, Miyagi M, Kamoda H, Takahashi K
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Journal Title
J Orthop Sci. 15
Pages: 402-6
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[Journal Article] Evaluation of behavior and neuropeptide markers of pain in a simple, sciatic nerve-pinch pain model in rats.2010
Author(s)
Hirose K, Iwakura N, Orita S, Yamashita M, Inoue G, Yamauchi K, Eguchi Y, Ochiai N, Kishida S, Nakamura J, Takaso M, Ishikawa T, Arai G, Miyagi M, Kamoda H, Aoki Y, Hiwatari R, Kakizaki J, Kunishi T, Kono M, Suzuki T, Toyone T, Takahashi K, Kuniyoshi K, Ohtori S.
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Journal Title
Eur Spine J. 19
Pages: 1746-52
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[Journal Article] Low back pain after lumbar discectomy in patients showing endplate modic type 1 change.2010
Author(s)
Ohtori S, Yamashita M, Yamauchi K, Inoue G, Koshi T, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Takahashi K
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Journal Title
Spine. 35
Pages: E596-600
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[Journal Article] Two-year results for scoliosis secondary to Duchenne muscular dystrophy fused to lumbar 5 with segmental pedicle screw instrumentation.2010
Author(s)
Takaso M, Nakazawa T, Imura T, Okada T, Toyama M, Ueno M, Fukushima K, Saito W, Minatani A, Miyajima G, Fukuda M, Takahira N, Takahashi K, Yamazaki M, Ohtori S, Okamoto H, Okutomi T, Okamoto M, Masaki T
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Journal Title
J Orthop Sci. 15
Pages: 171-7
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[Journal Article] Direct evidence for sensory innervation of the dorsal portion of the Co5/6 coccygeal intervertebral disc in rats.2010
Author(s)
Orita S, Ohtori S, Taniguchi A, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Eguchi Y, Ochiai N, Kishida S, Takaso M, Aoki Y, Takahashi K, Kawakami M
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Journal Title
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[Journal Article] Can the caudal extent of fusion in the surgical treatment of scoliosis in Duchenne muscular dystrophy be stopped at lumbar 5?2010
Author(s)
Takaso M, Nakazawa T, Imura T, Ueno M, Saito W, Shintani R, Takahashi K, Yamazaki M, Ohtori S, Okamoto M, Masaki T, Okamoto H, Okutomi T, Ishii K, Ueda Y
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Journal Title
Eur Spine J. 19
Pages: 787-96
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[Journal Article] Existence of pyogenic spondylitis in Modic type 1 change without other signs of infection : 2-year follow-up.2010
Author(s)
Ohtori S, Koshi T, Yamashita M, Yamauchi K, Inoue G, Suzuki M, Takaso M, Orita S, Eguchi Y, Ochiai N, Kishida S, Kuniyoshi K, Nakamura J, Aoki Y, Ishikawa T, Arai G, Miyagi M, Kamoda H, Takahashi K
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Journal Title
Eur Spine J. 19
Pages: 1200-5
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[Journal Article] Change in Modic Type 1 and 2 signals after posterolateral fusion surgery.2010
Author(s)
Ohtori S, Yamashita M, Yamauchi K, Inoue G, Koshi T, Suzuki M, Orita S, Eguchi Y, Ochiai N, Kishida S, Takaso M, Kuniyoshi K, Ishikawa T, Arai G, Miyagi M, Kamoda H, Nakamura J, Aoki Y, Takahashi K
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Journal Title
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[Journal Article] Surgical management of severe scoliosis with high risk pulmonary dysfunction in Duchenne muscular dystrophy : patient function, quality of life and satisfaction.2010
Author(s)
Takaso M, Nakazawa T, Imura T, Okada T, Fukushima K, Ueno M, Takahira N, Takahashi K, Yamazaki M, Ohtori S, Okamoto H, Okutomi T, Okamoto M, Masaki T, Uchinuma E, Sakagami H
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Journal Title
Int Orthop. 34
Pages: 695-702
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[Presentation] Takahashi K Disk injury produces transient increase of inflammatory cytokines in disks, whereas long-lasting increase painful neuropeptide in sensory nerve innervating disks in rats.2010
Author(s)
Miyagi M, Ishikawa T, Orita S, Eguchi Y, Kamoda H, Arai G, Suzuki M, Inoue G, Ohtori S
Organizer
Special Poster ISSLS (International Society for the Study of the Lumbar Spine)
Place of Presentation
Auckland, NZ
Year and Date
2010-04-10
