Effects of exercise-induced interleukin-6 secretion on protein synthesis in skeletal muscle

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500577
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Osaka University
• Principal Investigator: NAKAI Naoya Osaka University, 大学院・医学系研究科, 准教授 (90324508)
• Project Period (FY): 2008 – 2010
• Keywords: 機械的ストレッチ / タンパク質合成 / チロシンキナーゼ

Research Abstract

Murine myoblast cell line was mechanically stretched as a model for exercise-induced muscle contraction. Mechanical stretch increased the gene expression of interleukin-6 and activated the protein translation initiation. Stretch-induced activation of the protein translation initiation was completely blocked by tyrosin kinase inhibitor, genistein. Among the cellular protein which is activated by tyrosine kinase, phospholipase C was important for the signal transduction of mechanical stretch-induced activation of protein translation initiation.

Research Products

• [Journal Article] Effects of creatine and its analog, β-guanidinopropionic acid, on the differentiation of and nucleoli in myoblasts. (2011)
  • Author(s): Ohira, Y., Matsuoka, Y., Kawano, F., Ogura, A., Higo, Y., Ohira, T., Terada, M., Oke, Y., Nakai, N.
  • Journal Title: Biosci.Biotechnol.Biochem 印刷中
  • Peer Reviewed
• [Journal Article] Mechanical stretch activates signaling events for protein translation initiation and elongation in C2C12 myoblasts. (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R., Ohira, Y.
  • Journal Title: Mol.Cells. 30 Pages: 513-518
  • Peer Reviewed
• [Journal Article] Role(s) of gravitational loading during developing period on the growth of rat soleus muscle fibers. (2010)
  • Author(s): Kawano, F., Goto, K., Wang, XD., Terada,M., Ohira, T., Nakai, N., Yoshioka, T., Ohira, Y.
  • Journal Title: J.Appl.Physiol. 108(3) Pages: 676-685
  • Peer Reviewed
• [Journal Article] Myonucleus-related properties in soleus muscle fibers of mdx mice. (2010)
  • Author(s): Terada, M., Lan, Y.B., Kawano, F., Ohira, T., Higo, Y., Nakai, N., Imaizumi, K., Ogura, A., Nishimoto, N., Adachi, Y., Ohira, Y.
  • Journal Title: Cells Tissues Organs. 191 Pages: 248-259
  • Peer Reviewed
• [Journal Article] Effects of peroxisome proliferator-activated receptor α (PPARα) agonists on leucine-induced phosphorylation of translational targets in C2C12 cells. (2008)
  • Author(s): Nakai, N., Kawano, F., Terada, M., Oke, Y., Ohira, T., Ohira, Y.
  • Journal Title: Biochim.Biophys.Acta. 1780(10) Pages: 1101-1105
  • Peer Reviewed
• [Journal Article] Essential role of satellite cells in the growth of rat soleus muscle fibers. (2008)
  • Author(s): Kawano, F., Takeno, Y., Nakai, N., Higo, Y., Terada, M., Ohira, T., Nonaka, I., Ohira, Y.
  • Journal Title: Am.J.Physiol.Cell Physiol. 295(2) Pages: C458-C467
  • Peer Reviewed
• [Presentation] Tyrosine phosphorylation regulates mechanical stretch-induced activation of protein translation initiation in C2C12 myoblast. (2010)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Ohira, T., Fujita, R., Ohira, Y.
  • Organizer: Experimental Biology 2010.
  • Place of Presentation: Anaheim, California, USA
  • Year and Date: 20100424-20100428
• [Presentation] Protein translation initiation is activated by mechanical stretch in C2C12 myoblast. (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-20090801
• [Presentation] Mechanical stretch induces activation of eukaryotic elongation factor 2 in C2C12 myoblast. (2009)
  • Author(s): Nakai, N., Kawano, F., Oke, Y., Nomura, S., Terada, M., Ohira, T., Ohira, Y.
  • Organizer: Experimental Biology 2009.
  • Place of Presentation: New Orleans, Louisiana, USA
  • Year and Date: 20090418-20090422
• [Book] Muscle Cell Physiology (2009)
  • Author(s): Naoya Nakai, Yoshinobu Ohira
  • Total Pages: 136(3-14)
  • Publisher: Osaka University Press