Studies of food components having anti-oxidant effects on the 'depository gene' expression and its clinical application.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20500619
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied health science
• Research Institution: Kyushu University
• Principal Investigator: IKUYAMA Shoichiro Kyushu University, 生体防御医学研究所, 准教授 (20184393)
• Co-Investigator(Kenkyū-buntansha): OBA Koichi 九州大学, 病院, 助教 (50444857)
• Project Period (FY): 2008 – 2010
• Keywords: 生活習慣病 / 補完代替医療 / 健康食品 / 抗酸化 / 細胞内脂肪滴 / 遺伝子転写調節

Research Abstract

Excessive intracellular lipid accumulation causes various diseases such as atherosclerosis, fatty liver, etc. Specific proteins as we named 'depository genes' are involved in this process. We tested effects of an extract of French maritime pine, a widely used dietary supplement, on the expression of these genes in macrophages and liver cells. The compound suppressed the gene expression and lipid accumulation in both cells by different molecular mechanisms. We also analyzed the change in mRNA expression in white blood cells before and after ingestion of this compound using DNA microarray analysis. We identified 12 increased and 64 decreased genes, although physiological significance remains to be clarified at this moment.

Research Products

• [Journal Article] A Toll-like receptor 9-mediated pathway stimulates perilipin 3 (TIP47) expression and induces lipid accumulation in macrophages. (2010)
  • Author(s): Gu JQ, Wang DF, Yan XG, Zhong WL , Zhang J, Fan B, Ikuyama S.
  • Journal Title: Am J Physiol Endocrinol Meta 299 Pages: E593-E600
  • Peer Reviewed
• [Journal Article] Oleic acid-induced ADRP expression requires both AP-1 and PPAR-response elements, and is reduced by Pycnogenol through mRNA degradation in NMuLi liver cells. (2009)
  • Author(s): Fan B, Ikuyama S, Gu JQ, Wei P, Oyama J-I, Inoguchi T, Nishimura J.
  • Journal Title: Am J Physiol EndocrinolMetab 297 Pages: E112-E123
  • Peer Reviewed
• [Journal Article] Sixty percent of patients with rheumatoid arthritis in Japan have used dietary supplements or health foods. (2009)
  • Author(s): Ikuyama S, Imamura-Takase E, Tokunaga S, Oribe M, Nishimura J.
  • Journal Title: Modern Rheumatol 19 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Dietary supplement use in patients with rheumatoid arthritis in Japan. (2009)
  • Author(s): Ikuyama S, Imamura E.
  • Journal Title: Current Rheumatology Reviews 5 Pages: 241-245
  • Peer Reviewed
• [Journal Article] Pendrin is a novel autoantigen recognized by patients with autoimmune thyroid diseases. (2009)
  • Author(s): Yoshida A, Hisatome I, Taniguchi S, Shirayoshi Y, Yamamoto Y, Miake J, OhkuraT, Akama T, Igawa 0, Shigemasa C, Kamijo K, Ikuyama S, Caturegli P, Suzuki K.
  • Journal Title: J Clin Endocrinol Metab 94 Pages: 442-448
  • Peer Reviewed
• [Journal Article] Pycnogenol, an extract from French maritime pine, suppresses Toll-like receptor 4-mediated expression of adipose differentiation-related protein in macrophages. (2008)
  • Author(s): Gu JQ, Ikuyama S, Wei P, Fan B, Oyama J-I, Inoguchi T, Nishimura J.
  • Journal Title: Am J Physiol Endocrinol Metab 295 Pages: E1390-E1400
  • Peer Reviewed
• [Journal Article] Expression of adipose differentiation-related protein (ADRP) and perilipin in macrophages infected with Mycobacterium leprae. (2008)
  • Author(s): Tanigawa K, Suzuki K, Nakamura K, Akama T, Kawashima A, Wu H, Hayashi M, Takahashi S-I, Ikuyama S, Ito T, Ishii N.
  • Journal Title: FEMS Microbiol Lett 289 Pages: 72-79
  • Peer Reviewed
• [Presentation] Pycnogenol, an extract from French maritime pine, suppresses osteoclast differentiation. (2010)
  • Author(s): Ikuyama S, Wang Z, Fan B
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 20100326-20100330
• [Presentation] Function of adipose differentiation-related protein (ADRP) in myocardial steatosis (2010)
  • Author(s): Suzuki J, Ueno M, Kimura T, Imagawa M, Zenimaru Y, Takahashi S, Ikuyama S, Kraemer FB, Miyamori I
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 20100326-20100330
• [Presentation] ピクノジェノールによる肝細胞の細胞内脂肪滴形成の抑制 (2009)
  • Author(s): 萢斌、生山祥一郎、谷剣秋、井口登與志、西村純二
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20090521-20090524
• [Presentation] 心筋Adipose differentiation-related protein (ADRP)の機能 (2009)
  • Author(s): 鈴木仁弥、上野勝巳、木村朋子、今川美智子、銭丸康夫、高橋貞夫、生山祥一郎、宮森勇
  • Organizer: 第52回日本糖尿病学会年次学術集会(プレジデントポスターセッション)
  • Place of Presentation: 大阪
  • Year and Date: 20090521-20090524
• [Presentation] ピクノジェノールは肝細胞での細胞内脂肪滴蛋白ADRPの発現を抑制する (2009)
  • Author(s): 生山祥一郎、萢斌、西村純二
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋
  • Year and Date: 20090423-20090425
• [Presentation] ピクノジェノールはマクロファージにおけるTLR-4を介する細胞内脂肪滴蛋白ADRPの発現を抑制する (2008)
  • Author(s): 生山祥一郎、谷剣秋、西村純二
  • Organizer: 第11回日本補完代替医療学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20081108-20081109
• [Presentation] Pycnogenol^R, an extract from the bark of maritime pine, suppresses the expression of Toll-like receptor 4-mediated ADRP expression in macrophages. (2008)
  • Author(s): 生山祥一郎, 谷剣秋, 西村純二
  • Organizer: 90th Annual Meeting of The Endocrine Society
  • Place of Presentation: San Francisco, USA.
  • Year and Date: 20080615-20080618
• [Presentation] ピクノジェノールはマクロファージの細胞内脂肪滴蛋白ADRPの発現を抑制する (2008)
  • Author(s): 谷剣秋、生山祥一郎、西村純二
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 20080522-20080524
• [Presentation] 骨関節疾患患者におけるサプリメント利用の実態-薬剤性肝障害を発症した変形性膝関節症(OA)の1例 (2008)
  • Author(s): 西川寛、生山祥一郎、大庭功一、高瀬恵美子、西村純二
  • Organizer: 第18回日本老年医学会九州地方会
  • Place of Presentation: 福岡
  • Year and Date: 2008-03-01
• [Remarks] ホームページ等