2010 Fiscal Year Final Research Report
Studies on the biochemical mechanism of N_2O generation by marine nitrifying bacterium
| Project/Area Number |
20510007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | Shizuoka University |
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Principal Investigator |
FUJIWARA Taketomo Shizuoka University, 理学部, 教授 (80209121)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 地球温暖化 / 亜酸化窒素 |
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| Research Abstract |
Hydroxylamine oxidoreductase (HAO) was purified from marine ammonia-oxidizing bacterium (AOB) strain NS58. Generation of N_2O as a by-product of HAO reaction was evidenced by in vitro experiment using the purified enzyme. Unique isotopic property of the N_2O generated by HAO reaction was also revealed. Molecular and enzymatic features of nitrite reductase, that was involved in the nitrifier denitrification pathway (another N_2O generation mechanism in AOB), was investigated using the recombinant enzyme.
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