Genetic interaction between Rad18 and checkpoint factors

2011 Fiscal Year Final Research Report

• Project/Area Number: 20510053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kumamoto University
• Principal Investigator: TATEISHI Satoshi 熊本大学, 発生医学研究所, 講師 (00227109)
• Project Period (FY): 2008 – 2011
• Keywords: Rad18 / Chk2 / チェックポイント / 細胞老化

Research Abstract

Rad18 is required for long-term maintenance of spermatogenesis in mouse testes. We prepared fibroblasts from Chk2-Rad18 double knockout mice. The cells grew very slowly and exhibited cellular senescence. The Cdk inhibitors such as p16, p21 and p53 proteins were highly expressed in the cells. Many RPA protein foci were observed in the nuclei of the cells, thus we inferred that loss of Chk2 and Rad18 resulted in activation of ATR pathway and expression of Cdk inhibitors leading to low cellular growth and senescence.

Research Products

• [Journal Article] A novel Rad18 ubiqitin ligase-mediated pathway for repair of camptothecin-induced DNA damage (2011)
  • Author(s): Tateishi, S.
  • Journal Title: Cell Cycle Volume: 10 Pages: 2057-2058
  • Peer Reviewed
• [Journal Article] NBS1 recruits RAD18 via a RAD6-like domain and regulates Pol-dependent translesion DNA synthesis (2011)
  • Author(s): Yanagihara, H., Kobayashi, J., Tateishi, S., Kato, A., Matsuura, S., Tauchi, H., Yamada, K., Takezawa, J., Sugasawa, K., Masutani, C., Hanaoka, F., Weemaes, C. M., Mori, T., Komatsu, K.
  • Journal Title: Mol. Cell Volume: 43 Pages: 788-797
  • Peer Reviewed
• [Journal Article] PCNA ubiquitination is important, but not essential for translesion DNA synthesis in mammalian cells (2011)
  • Author(s): Hendel, A., Krijger, P. H., Diamant, N., Goren, Z., Langerak, P., Kim, J., ReiBner, T., Lee, K. Y., Geacintov, N. E., Carell, T., Myung, K., Tateishi, S., D' Andrea, A., Jacobs, H., Livneh, Z.
  • Journal Title: PLoS Genet Volume: 9 Pages: e1002262
  • Peer Reviewed
• [Journal Article] The vital role of polζand REV1 in mutagenic, but not correct, DNA synthesis across benzo[・] pyrene-dG and the recruitment of polζby REV1 to a replication-stalled site
  • Author(s): Hashimoto, K., Cho, Y., Yang, I., Akagi, J., Ohashi, E., Tateishi, S., Wind, N., Hanaoka, F., Ohmori, H., Moriya, M.
  • Journal Title: J. Biol. Chem Volume: (In press)
  • Peer Reviewed
• [Journal Article] Exome sequencing identifies KIAA1530 as the causal gene for UV sensitive syndrome complementation group A(UVsS-A)
  • Author(s): Nakazawa, Y., Sasaki, K., Mitsutake, N., Matsuse, M., Shimada, M., Ohyama, K., Ito, K., Masuyama, R., Kudo, T., Utani, A., Takenaka, K., Miki, Y., Nardo, T., Stefanini, M., Takahashi, Y., Yamashita, S., Tateishi, S., Lehmann, A., Yoshiura, K., Ogi, T.
  • Journal Title: Nat. genet Volume: (In press)
  • Peer Reviewed
• [Presentation] Loss of Rad18 alleles suppresses tumorigenesis in p53-null mice via cellular senescence (2012)
  • Author(s): Tateishi, S.
  • Organizer: Gordon conference DNA Damage Mutation & Cancer
  • Place of Presentation: Ventura, U. S. A.
  • Year and Date: 20120325-30
• [Presentation] Rad18 is required for double-stranded break repair and long-term spermatogenesis (2011)
  • Author(s): Tateishi, S., Watanabe, K., Sun, J., Iwabuchi, K., Yomogida, K.
  • Organizer: 第34回日本分子生物学会年会ワークショップMolecular mechnism of replication fork recovery pathways
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 20111213-16
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/cell_genetics/index.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/en/divisions/Cell_Genetics/index.html
• [Remarks]
  • URL: http://www.cockayneresearchcare.jp/idea/journal5.html