2010 Fiscal Year Final Research Report
Visualization of protein molecules on single cells with surface-enhanced-Raman active nano-particles
| Project/Area Number |
20510111
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
ISHIKAWA Mitsuru National Institute of Advanced Industrial Science and Technology, 健康工学研究部門, 研究グループ長 (70356434)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Tamitake 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (00351742)
ABE Hiroko 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (40363220)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ナノ粒子・ナノチューブ / バイオイメージング |
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| Research Abstract |
The purpose of the current project is in-situ detection of protein molecules on the surface of a single cell in a highly-sensitive manner. Key proteins on the surface will appear and disappear within several minutes in a cell cycle. Thus, measurement much faster than that by conventional Raman spectroscopy is highly required. We select surface-enhanced Raman spectroscopy (SERS) to meet the requirement. We observed a cell cycle of a single yeast cell as a model system with the help of SERS from a protein in a non-invasive manner. Furthermore, the protein is successfully identified by SERS spectroscopy and inhibition assays for the protein.
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[Journal Article] Selective Detection of HbA1c using Surface Enhanced Resonance Raman Spectroscopy2010
Author(s)
M.S.Kiran, Itoh, T., Yoshida, K., Kawashima, N., Biju, V., Ishikawa, M.
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Journal Title
Anal.Chem. 82
Pages: 1342-1348
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