2010 Fiscal Year Final Research Report
Development of in vitro toxicity methods for fire combustion products by using an artificial lung and tissue cells
| Project/Area Number |
20510167
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social systems engineering/Safety system
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| Research Institution | Oita National College of Technology |
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Principal Investigator |
KONISHI Tadashi Oita National College of Technology, 機械工学科, 教授 (00225468)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 火災ガス / シアン化水素 / 毒性評価 / 非動物実験 / ヒト肺胞基底上皮細胞 / A549 |
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| Research Abstract |
The toxic valuation method of the fire gas by the human alveolus basal epithelium cells A549 was established as an alternative method of animal experiments, such as rat and mouse. The toxic assessment system of fire gas consists of fire gas collection equipment, exposure equipment, and toxic evaluation equipment. In the ABS resin experiment, the number of initial cells ; 3x10^5 cells/ml increased to 3x10^6 cells/ml after three-day fire gas exposure, and survival ratio was 95% under 5%-CO_2environment. It increased to 1x10^6 cells/ml and survival ratio was 90% under atmospheric environment. It decreased to 5x10^4 cells/ml and survival ratio became 20% under fire gas environment.
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