2010 Fiscal Year Final Research Report
Regulation assay of light dependent transcription using a cell-free system derived from Arabidopsis
| Project/Area Number |
20510188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Nagoya City University |
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Principal Investigator |
YUKAWA Yasushi Nagoya City University, 大学院・システム自然科学研究科, 教授 (70381902)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 光依存 / 転写制御 / シロイヌナズナ / 無細胞 / 遺伝子発現 |
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| Research Abstract |
Two Cell-free transcription assay systems were established from rapidly growing Arabidopsis cultured cell MM2d line. The cultured cells were etiolated when the cells were growing under dark condition and were greening when the cells were growing under light condition. Using these two different colored cells, in vitro transcription systems were established. And then, using the two in vitro transcription systems, we have successfully assayed light dependent transcription regulation using Arabidopsis ribulose-1,5-bis-phosphate carboxylase/oxygenase (rbcS) genes.
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