Development of innovative genetic manipulation methods of Bifidobacterium based on the genome information.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20510189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Gifu University
• Principal Investigator: SUZUKI Tohru Gifu University, 連合農学研究科, 教授 (20235972)
• Project Period (FY): 2008 – 2010
• Keywords: 形質転換 / プラスミド人工修飾法 / PAM / 遺伝子破壊 / Bifidobacterium adolescentis / 相同組換え / 逆遺伝学 / Bifidobacterium longum

Research Abstract

Bifidobacteria are thought to be important bacteria for human health. However, the scientific evidences has not yet obtained because it has been difficult to manipulate the gene. In this study, we developed new transformation method, selection marker and gene knock-out method based on the genome analyses.

Research Products

• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate. (2011)
  • Author(s): Fukuda, S., H.Toh, K.Hase, K.Oshima, Y.Nakanishi, K.Yoshimura, T.Tobe, J.M.Clarke, D.L.Topping, T.Suzuki, T.D.Taylor, K.Itch, J.Kikuchi, H.Morita, M.Hattori, H.Ohno.
  • Journal Title: Nature 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces. (2010)
  • Author(s): Yokoyama, S., T.Niwa, T.Osawa, T.Suzuki.
  • Journal Title: Arch.Microbial.(DOI 10.1007/s00203- 009-0524-5) 192 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Intra-species Diversity Between Seven Bifidobacterium adolescentis Strains Identified by Genome-wide Tiling Array Analysis. (2009)
  • Author(s): Yasui, K., M.Tabata, S.Yamada, T.Abe, T.Ikemura, R.Osawa, T.Suzuki.
  • Journal Title: Biosci.Biotechnol. Biochem. 73 Pages: 1422-1424
  • Peer Reviewed
• [Journal Article] Improvement of bacterial transformation efficiency using plasmid artificial modification. (2009)
  • Author(s): Yasui, K., Y.Kano, K.Tanaka, K.Watanabe, M.Shimizu-Kadota, H.Yoshikawa, T.Suzuki.
  • Journal Title: Nucleic Acids Res doi:10.1093/nar/gkn884 Pages: 37
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a novel equol-producing bacterium from human feces. (2008)
  • Author(s): Yokoyama, S.-I., T.Suzuki.
  • Journal Title: Biosci.Biotechnol.Biochem. 72 Pages: 2660-2666
  • Peer Reviewed
• [Presentation] Expression of catalase gene affords toleranceto oxygen in Bifidobacterium longum 105 A. (2010)
  • Author(s): Jianlong, H., T.Suzuki
  • Organizer: in 3rd Intnatinal Symposium on Propionibacteria and Bifidobacteria.
  • Place of Presentation: Oviedo, Spain.
  • Year and Date: 20100000
• [Presentation] Construction and molecular characterization of the temperature sensitive plasmid for Bifidobacterium longum. (2010)
  • Author(s): 坂口広太, 鈴木徹
  • Organizer: in 3rd Intnatinal Symposium on Propionibac teria and Bifidobacteria.
  • Place of Presentation: Oviedo, Spain.
  • Year and Date: 20100000
• [Presentation] ダイズイソフラボンをEquolに変換するEggerthella sp.YY7918のゲノム解析 (2010)
  • Author(s): 横山慎一郎, 大島健志朗, 野村泉, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キヤンパス
  • Year and Date: 20100000
• [Presentation] ダイズイソフラボンをO-DMAに変換するヒト腸内由来の新規Clostridium Cluster XIVa細菌SY8519株のゲノム解析 (2010)
  • Author(s): 横山慎一郎, 野村泉, 鈴木徹, 大島健志朗, 服部正平
  • Organizer: in日本乳酸菌学会2010年度大会
  • Place of Presentation: フォレスト仙台
  • Year and Date: 20100000
• [Presentation] 牛乳ラクトフォリン(milk Glycam-1)のN型糖鎖構造の多様性 (2010)
  • Author(s): 稲垣瑞穂, 中家修一, 野原大輔, 矢部富雄, 金丸義敬, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 20100000
• [Presentation] Bifidobacterium longumで機能するプロモーターの活性比較 (2010)
  • Author(s): 賀建龍, 鈴木徹
  • Organizer: in農芸化学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 20100000
• [Presentation] プラスミ人工修飾法(PAM)によるビフィズス菌の遺伝子導入系の開発 (2009)
  • Author(s): 安井一将, 坂口広太, 加納康正, 門多真理子, 吉川博文, 鈴木徹
  • Organizer: in第13回腸内細菌学会
  • Place of Presentation: 北里大学薬学部,東京.
  • Year and Date: 20090000
• [Presentation] Aspergillus oryzae のキシリトール代謝関連遺伝子削除株によるキシリトール生産 (2009)
  • Author(s): 榊原由佳, 渥美元規, 中村浩平, 北本則行, 鈴木徹, 高見澤一裕
  • Organizer: in生物工学会61回大会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 20090000
• [Presentation] 枯草菌を用いたPAM(Plasmid Artificial Modification)法による乳酸球菌Lactococcus lactis IO-1株の形質転換頻度の改善 (2009)
  • Author(s): 渡辺寛, 廣崎辰也, 東崎正, 鈴木徹, 園元謙二, 門多真理子, 吉川博文.
  • Organizer: in日本乳酸菌学会2009年度大会
  • Place of Presentation: ベルクラシック甲府
  • Year and Date: 20090000
• [Presentation] A repeatable gene disruption method for Bifidobacterium longum 105-A (2008)
  • Author(s): Sakaguchi K., N.Funaoka, Y.Kano, T.Suzuki
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria
  • Place of Presentation: Egmond, Netherlands : FEMS.
  • Year and Date: 20080000
• [Presentation] Complete genome structure of Bifidobacterium adolescentis ATCC15703 and inter strain genome-wide polymorphism between 7 B.adolescentis strains using tilling array analyses. (2008)
  • Author(s): Suzuki T., H.Tabata, K.Suganuma, K.Yasui, K.Tanaka, K.Watanabe, K.Sakaguchi, T.Abe, T.Ikemura, R.Osawa
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria.
  • Place of Presentation: Egmond, Netherlands : FEMS.
  • Year and Date: 20080000
• [Presentation] Plasmid artificial modification (PAM) : a genomic information-based method to improve transformation efficiency in Bifidobacterium adolescentis ATCC15703. (2008)
  • Author(s): 安井一将, 加納康正, 鈴木徹
  • Organizer: in 9th Symposoum on Lactic Acid Bacteria.
  • Place of Presentation: Egmond Netherlands : FEMS.
  • Year and Date: 20080000
• [Presentation] プラスミド人工的メチル化(PAM)によるBifidobacterium adolescentis ATCC15703の形質転換効率の向上 (2008)
  • Author(s): 安井一将, 鈴木徹, 加納康正
  • Organizer: in日本乳酸菌学会2008年度大会
  • Place of Presentation: 京都大学百周年時計台記念館
  • Year and Date: 20080000
• [Presentation] ヒト糞便由来の新規Equol産生菌の単離と諸性質について (2008)
  • Author(s): 横山慎一郎, 鈴木徹
  • Organizer: in生物工学会60回大会
  • Place of Presentation: 東北学院大学土樋キャンパス
  • Year and Date: 20080000
• [Presentation] タイリングアレイと自己組織化マップを用いたBifidobacterium adolescentis 菌株間のゲノム多型の網羅的解析 (2008)
  • Author(s): 鈴木徹, 田端大嗣, 菅沼宏美, 安井一将, 田中香お里, 渡邉邦友, 池村淑道, 阿部貴志, 大澤朗.
  • Organizer: in第12回腸内細菌学会
  • Place of Presentation: 東京大学弥生講堂
  • Year and Date: 20080000
• [Book] 染色体 DNA の遺伝解析.世紀を超えるビフィズス菌の研究 その基礎と臨床応用から 製品開発へー (2011)
  • Author(s): 鈴木徹
  • Publisher: 日本ビフィズス菌センタ ー
• [Book] Strain Engineering : Methods and Protocols, Methods in Molecular Biology(Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria.)(In J.A.Williams (ed.)) (2011)
  • Author(s): Suzuki T, K.Yasui
  • Publisher: A Humana Press.
• [Book] Lactic Acid Bacteria : Sophisticated Progress(Current status of Bifidobacterium gene maniplation technology.)(In K.Sonomoto and A.Yokota (ed.)) (2010)
  • Author(s): Fukiya, S., Suzuki T, Y.Kano, A.Yokota.
  • Publisher: Hrizon Scientific Press, UK.
• [Remarks] ホームページ等