2010 Fiscal Year Final Research Report

Search of natural compound activating endogenous anti-angiogenesis factor as an anti-cancer agent.

Research Project

Project/Area Number	20510209
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Living organism molecular science
Research Institution	Hyogo University of Health Sciences
Principal Investigator	AOKI Shunji Hyogo University of Health Sciences, 薬学部, 教授 (60252699)
Co-Investigator(Renkei-kenkyūsha)	FURUKAWA Tatsuhiko 鹿児島大学, 大学院・医歯学総合研究科, 教授 (40219100)
Project Period (FY)	2008 – 2010
Keywords	内因性血管新生阻害因子 / プロモーター活性化 / thrombospondin-1 / ヒト齊帯静脈血管内皮細胞 / 腫瘍血管新生
Research Abstract	In this study, we attempt to find the natural compounds which activate the promoter of "endogenous anti-angiogenesis factor" such as endostatin, angiostatin and thrombospondin. We tried to establish a bioassay method using the cells that were stably transfected by the luciferase reporter plasmid fused with the promoter domain of the thrombospondin-1 gene. However, We couldn't achieve to establish the stably transfected cells. On the other hand, on the guidance of selective growth inhibition against human umbilical vein endothelial cells (HUVECs), we searched for anti-angiogenic substances from extracts of medicinal plants. We found several active extract and tried to isolate the active components by the assay-guided separation.