2010 Fiscal Year Final Research Report
Crystallization of Proteins based on Molecular Assembling by Photon Force
| Project/Area Number |
20550002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
TSUBOI Yasuyuki Hokkaido University, 大学院・理学研究院, 准教授 (00283698)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 光圧 / 輻射力 / 放射圧 / 結晶化 / プラズモン / 分子集合体 / アミノ酸 / リゾチーム |
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| Research Abstract |
We demonstrated a novel technique to promote crystallization of hen egg-white lysozyme in heavy water. The technique was essentially based on aggregation of the protein molecules by photon pressure generated under irradiation of a cw 1064 nm laser beam to the protein solution. When photon pressure was kept being exerted for 1-2 hours on the sample solution, a small particle identified to the molecular aggregate of lysozyme was produced, as proved by in situ Raman microspectroscopy on the particle. Furthermore, we confirmed that aggregation of lysozyme by photon pressure led to crystallization of the protein.
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