2010 Fiscal Year Final Research Report
Development of High-Performance Two-Dimensional Electrophoresis Using Low-Concentration Gel and Its Application
| Project/Area Number |
20550072
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ODAKE Tamao 東京大学, 大学院・工学系研究科, 研究員 (10301128)
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| Co-Investigator(Kenkyū-buntansha) |
TSUNODA Kin-ichi 群馬大学, 大学院・工学研究科, 教授 (30175468)
SAHEKI Toshihiko 群馬大学, 大学院・工学研究科, 助教 (00241860)
UEKI Yuji 日本原子力研究開発機構, 量子ビーム応用研究部門, 研究員 (50446415)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分離分析 / 電気泳動 |
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| Research Abstract |
The researchers have developed a new isoelectric focusing(IEF) gel supported by a multifilament as a first-dimensional IEF gel of two-dimensional electrophoresis(2-DE), which can separate proteins with high-resolution. The multifilament-supporting(MFS) gel could overcome difficulty in handling the IEF gel, prepare low-concentration gel to separate high-molecular-weight proteins. In this study, to improve operability and reproducibility, support of the low-concentration gel, method of polymerization, and introduction of immobilized pH gradient were investigated. The 2-DE system using the low-concentration MFS gel was proved to be useful for proteome analysis.
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