2010 Fiscal Year Final Research Report
Specific degradation of peptides with hydrogen radical and its application to protein chemistry
| Project/Area Number |
20550081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
TAKAYAMA Mitsuo Yokohama City University, 生命ナノシステム科学研究科, 教授 (10328635)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 水素ラジカル / 質量分析 / タンパク質 |
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| Research Abstract |
The ultrasound sonolytic hydrolysis of peptides was performed with added benzenethiol as a hydrogen-releasing reagent in aqueous solution. The ultrasound hydrolysis of peptides differed from that due to conventional acid hydrolysis in characteristics, i.e., cleavage at proline residue and dehydration as a side reaction. The sonolysis combined with the thiol addition made it possible to determine the amino-acid sequence of peptides. Furthermore, new reagent for releasing hydrogen atoms was found out in the experiment of the irradiation of ultraviolet laser photon in vacuum condition.
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