Synthesis of Cello-Oligosaccharides for Alcohol Fermentation by Hydrolysis of Crystalline-Region Cellulose

2010 Fiscal Year Final Research Report

• Project/Area Number: 20550116
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Polymer chemistry
• Research Institution: Kochi University of Technology
• Principal Investigator: TOSHIYUKI Uryu Kochi University of Technology, 工学部, 副学長 (80011005)
• Co-Investigator(Kenkyū-buntansha): TAKASHI Yoshida 北見工業大学, 工学部, 副学長
• Project Period (FY): 2008 – 2010
• Keywords: セルロース / 結晶領域セルロース / 高温高圧処理 / 水蒸気爆砕 / エタノール / 酸加水分解 / 結晶構造変化 / 酵素分解

Research Abstract

A practical method to hydrolyze crystalline cellulose was found out. After crystalline cellulose suspension in water was heated at 260℃±10℃ in which temperature the hydrogen bond is weakened, it was steam-exploded. The obtained cellulose showed that hydrolysis occurred only at its surface. Mercerized cellulose showed the same phenomenon. For the crystalline cellulose converted into a different crystalline state, an acidic hydrolysis around 200℃ gave mono- and oligo-saccharide derivatives, indicating the occurrence of main-chain scission. The converted cellulose was easily hydrolyzed by enzymes into monoand oligo-saccharides.

Research Products

• [Journal Article] Two-Step Cellulose Hydrolysis Using Modest Amount of Concentrated Sulfuric Acid or Autoclave and Fermentation of the Hydrolyzates into Ethanol Utilizing Recombinant Yeast (2010)
  • Author(s): T. Uryu, K. Katsuraya, K. Okuyama, H. Kato, C. Uchiyama, A. Oka, N. Kubo, M. Takahashi, M. Kudo, M. Kawabe, T. Kobayashi, S. Ohnishi, M. Aida, K. Shishikura, H. Iefuji, K. Kobiro, Y. Tsutsui, T. Yoshida
  • Journal Title: Bulletin of Kochi University of Technology 7 Pages: 101-108
• [Journal Article] Sulfated Oligosaccharide Cluster with Polylysine Core Scaffold as a New Anti-HIV Dendrimer (2010)
  • Author(s): S. Han, D. Yoshida, T. Kanamoto, H. Nakashima, T. Uryu, T. Yoshida
  • Journal Title: Carbohydr.Polym. 80 Pages: 1111-1115
• [Journal Article] Novel Application of Electrooxidative Method for the Cyclization of N-Benzyl-2-(hydroxyl-methyl)-and N-benzyl-2-(2-hydroxylethyl)-piperidines (2010)
  • Author(s): M. Okimoto, T. Yoshida, M. Hoshi, K. Ohashi
  • Journal Title: Heterocycles 81 Pages: 2471-2478
• [Journal Article] Effects of Lacquer Polysaccharides, Glycoproteins and Isoenzymes on the Activity of Free and Immobilized Laccase fromRhus vernicifera (2010)
  • Author(s): Y. Wan, R. Lu, K. Akiyama, K. Okamoto, T. Honda, Y. Du, T. Yoshida, T. Miyakoshi, C. J. Knill, J. F. Kennedy
  • Journal Title: Intern.J.Biol.Macromol. 47 Pages: 76-81
• [Journal Article] Synthesis and Specific Influenza A Virus-Adsorptive Functionality of Alkyl Curdlan Sulfate-Coated Membrane Filter
  • Author(s): M. Tegshi, S. Han, T. Kanamoto, H. Nakashima, T. Yoshida
  • Journal Title: J.Polym.Sci.Part A : Polym.Chem., submitted
• [Presentation] 形質転換酵母によるセロオリゴ糖からのバイオエタノール合成 (2011)
  • Author(s): 梁鮮香、吉田孝、瓜生敏之
  • Organizer: 第45回高分子学会北海道支部研究発表会
  • Place of Presentation: 札幌(ポスター)
  • Year and Date: 2011-01-31
• [Presentation] セルロースの前処理と糖化、発酵 (2011)
  • Author(s): 梅田篤、森文香、沖本光宏、石澤真也、吉田孝、瓜生敏之
  • Organizer: 第45回高分子学会北海道支部研究発表会
  • Place of Presentation: 札幌(ポスター)
  • Year and Date: 2011-01-31
• [Presentation] Direct Ethanol Fermentation of Cello-oligosaccharides by Recombinant Yeast pYBGA1 (2010)
  • Author(s): 梁鮮香, 吉田孝, 瓜生敏之
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu(ポスター)
  • Year and Date: 2010-12-17
• [Presentation] 形質転換酵母によるセロオリゴ糖のエタノール発酵条件の検討 (2010)
  • Author(s): 梁鮮香、吉田孝、瓜生敏之
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌(ポスター)
  • Year and Date: 2010-09-16
• [Presentation] セルロースのセロオリゴ糖への前処理法の検討 (2010)
  • Author(s): 梅田篤、吉田孝、瓜生敏之
  • Organizer: 第59回高分子討論会
  • Place of Presentation: 札幌(ポスター)
  • Year and Date: 2010-09-16
• [Presentation] セロオリゴ糖の直接発酵によるバイオエタノールの合成 (2010)
  • Author(s): 梁鮮香、吉田孝
  • Organizer: 2010年度日本化学会北海道支部夏季研究発表会
  • Place of Presentation: 函館(口頭発表)
  • Year and Date: 2010-07-24
• [Presentation] 遺伝子組換酵母によるセルロース系バイオエタノールの合成 (2010)
  • Author(s): 梅田篤、森文香、沖本光宏、石澤真也、吉田孝、瓜生敏之
  • Organizer: 日本化学会北海道支部夏季研究発表会
  • Place of Presentation: 函館(口頭発表)
  • Year and Date: 2010-07-24
• [Presentation] セロオリゴ糖の直接発酵によるバイオエタノールの合成 (2010)
  • Author(s): 梁鮮香、吉田孝、瓜生敏之
  • Organizer: 第59回高分子学会年次大会
  • Place of Presentation: 横浜(ポスター)
  • Year and Date: 2010-05-23