2010 Fiscal Year Final Research Report
Rational Design of Gene-Therapeutic Ribozyme
| Project/Area Number |
20550145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tohoku University |
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Principal Investigator |
TANAKA Yoshiyuki Tohoku University, 大学院・薬学研究科, 准教授 (70333797)
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| Co-Investigator(Renkei-kenkyūsha) |
KATO Yoshio 独立行政法人産業技術総合研究所, セルエンジニアリング研究部門, 研究員 (20415657)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 核酸 / 蛋白質 / 糖化学 |
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| Research Abstract |
Gene therapy using hammerhead ribozyme were studied. It is known that hammerhead ribozyme can only cleave RNAs with UC or UA or UU sequence near the cleavage site. Based on the crystal structure of the hammerhead ribozyme, we drastically expanded the variations of the cleavable sequences, in a logical and rational manner (base-substitutions or a non-standard base-introduction into the hammerhead ribozyme). These modified ribozymes with wider cleavable sequences could be useful for a gene therapy.
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