On-chip cell-free genetic engineering as a research technology for proteomics

2010 Fiscal Year Final Research Report

• Project/Area Number: 20550148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: The University of Tokyo
• Principal Investigator: NOJIMA Takahiko The University of Tokyo, 一般教育部, 講師 (00291930)
• Co-Investigator(Renkei-kenkyūsha): FUJII Teruo 東京大学, 生産技術研究所, 教授 (30251474)
• Project Period (FY): 2008 – 2010
• Keywords: DNA / GFP / PCR / キャピラリー電気泳動 / タンパク質 / プロテオミクス / 翻訳

Research Abstract

An experimental system has been developed to achieve recombinant DNA and gene expression without living cell by enzymatic reactions and on-chip electrophoresis of DNA.

Research Products

• [Journal Article] Electrophoresis (2010)
  • Author(s): Shohei Kaneda, Koichi Ono, Tatsuhiro Fukuba, Takahiko Nojima, Takatoki Yamamoto, Teruo Fujii
  • Journal Title: Vol.31, No.22 Pages: 3719-3726
  • Peer Reviewed
• [Journal Article] Takahiko Nojima, Teruo Fujii Hiroyuki Fujita (2008)
  • Author(s): Hideyuki F. Arata, Frederic Gillot,
  • Journal Title: Lab on a Chip Vol.8, No.9 Pages: 1436-1440
  • Peer Reviewed
• [Journal Article] Polymerase chain reaction-based molecular logic gate coupled with cell-free transcription-translation as a reporter (2008)
  • Author(s): Takahiko Nojima, Takatoki Yamamoto, Hiroshi Kimura, Teruo Fujii
  • Journal Title: Chemical Communications No.32 Pages: 3771-3773
  • Peer Reviewed
• [Journal Article] Cell-free protein synthesis conducted by template DNA with repetitive sequence, Chemistry (2008)
  • Author(s): Takahiko Nojima, Hiroshi Kimura, Teruo Fujii
  • Journal Title: Letters Vol.37, No.6 Pages: 648-649
  • Peer Reviewed
• [Presentation] DNA増幅反応と生体外蛋白質合成を組み合わせた分子論理ゲートシステムの構築 (2011)
  • Author(s): 野島高彦
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学横浜キャンパス
  • Year and Date: 20110326-20110329
• [Presentation] PCR-based biomolecular logic gate (2010)
  • Author(s): 野島高彦
  • Organizer: 2010International Chemical Congress of Pacific Basin Societies(Pacifichem2010)
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20101115-20101120
• [Presentation] Fluorescent green logic (2008)
  • Author(s): 野島高彦
  • Organizer: 第11回生命化学研究会～生命化学をシステムで捉えたら～,水上館
  • Place of Presentation: 群馬県みなかみ町
  • Year and Date: 20081128-20081129
• [Presentation] 同一遺伝子を繰り返しコードした鋳型DNAからの無細胞蛋白質合成 (2008)
  • Author(s): 野島高彦
  • Organizer: 第57回高分子討論会
  • Place of Presentation: 大阪市立大
  • Year and Date: 20080924-20080926
• [Presentation] PCRに基づく生化学ロジックゲート (2008)
  • Author(s): 野島高彦
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京工業大学すずかけ台キャンパス
  • Year and Date: 20080918-20080920
• [Presentation] DNA増幅と遺伝子発現にもとづくロジックゲート構築のこころみ (2008)
  • Author(s): 野島高彦
  • Organizer: 第17回化学とマイクロ・ナノシステム研究会(17thCHEMINAS)
  • Place of Presentation: 九州大学馬出キャンパス
  • Year and Date: 20080520-20080521
• [Remarks] 研究の一部が英国王立化学会刊行のChemical Science 紙の第一面で紹介された.2008年第8号1面,Jellyfish protein glows to demonstrate logic gate success/Fluorescent green logic
  • URL: http://www.rsc.org/Publishing/ChemScience/Volume/2008/08/Fluorescentgreen_logic.asp
• [Remarks] 研究内容は代表者のホームページにて紹介している
  • URL: http://www.takahiko.info/