2010 Fiscal Year Final Research Report
Preparation of the glycol-arrays for aiming at the ordered and well-defined accumulation and the analyses of the interaction to proteins
| Project/Area Number |
20550150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MORI Toshiaki Tokyo Institute of Technology, 大学院・生命理工学研究科, 准教授 (50262308)
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| Project Period (FY) |
2008 – 2010
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| Keywords | atomic force microscopy (AFM) / verotoxin / Gb3 sugar molecules / single molecular measurement / Quartz-crystal microbalance (QCM) |
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| Research Abstract |
In order to elucidate the multivalent interactions between carbohydrates and proteins, and to prepare the glycol arrays which immobilized onto the solid surface
We could succeed in arraying carbohydrate ligands on the surface with dispersion by utilizing the biotin-avidin interaction, and the kinetic analyses for protein binding could be performed with the QCM system.
Moreover, we have analyzed interaction forces between a verotoxin that is a substance responsible for o-157 and Gb3 sugar molecules on a cell surface. The rupture forces were obtained by changing loading rates and we can get an effective bond length which is an important parameter for an analysis of interaction mechanisms. We can have detected single-molecular interaction forces, and we will discuss about the interaction mechanism in more detail by an analysis of the forces.
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