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2010 Fiscal Year Final Research Report

Construction and Direct Observation of Molecular Assembly of Photosynthetic Membrane Proteins

Research Project

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Project/Area Number 20550151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionNagoya Institute of Technology

Principal Investigator

DEWA Takehisa  Nagoya Institute of Technology, 大学院・工学研究科, 准教授 (70335082)

Co-Investigator(Renkei-kenkyūsha) NANGO Mamoru  名古屋工業大学, 大学院・工学研究科, 教授 (90109893)
Research Collaborator SUGIURA Ryuta  名古屋工業大学, 大学院・工学研究科, 博士前期課程
TAKEUCHI Toshikazu  名古屋工業大学, 大学院・工学研究科, 博士前期課程
HIRO Akito  名古屋工業大学, 大学院・工学研究科, 博士前期課程
SUMINO Ayumi  名古屋工業大学, 大学院・工学研究科, 博士後期課程
SASAKI Nobuaki  名古屋工業大学, 大学院・工学研究科, 博士前期課程
WATANABE Natsuko  名古屋工業大学, 大学院・工学研究科, 博士前期課程
Project Period (FY) 2008 – 2010
Keywords光合成 / 膜タンパク質 / 集合構造 / 脂質二分子膜 / 再構成 / 原子問力顕微鏡 / 光エネルギー変換
Research Abstract

Photosynthetic membrane proteins (LH2 and LH1-RC) that play important roles of light harvesting and charge separation were successfully reconstituted into lipid bilayer membrane to investigate the relationship between the assembly structure and their function. The structure of the assembly consisting of LH2 and LH1-RC were clearly observed by atomic force microscopy at the molecular level. The structure likely correlates the function, e.g., energy transfer from LH2 to LH1-RC. The basic findings should provide useful information for highly efficient construction of photoenergy conversion system.

  • Research Products

    (23 results)

All 2011 2010 2009 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (13 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Selective Assembly of Photosynthetic Antenna Proteins into a Domain-Structured Lipid Bilayer for the Construction of Artificial Photosynthetic Antenna Systems : Structural Analysis of the Assembly using Surface Plasmon Resonance and Atomic Force Microscopy2011

    • Author(s)
      Sumino, Dewa, (他6名)
    • Journal Title

      Langmuir 27

      Pages: 1092-1099

    • Peer Reviewed
  • [Journal Article] Reconstitution and AFM Observation of Photosynthetic Membrane Protein Assembly in Planar Lipid Bilayers2011

    • Author(s)
      Sumino, Dewa (他6名)
    • Journal Title

      e-J.Surf.Sci.Nanotech. 9

      Pages: 15-20

    • Peer Reviewed
  • [Journal Article] Reconstitution of bacterial photosynthetic unit in lipid bilayer studied by single-molecule spectroscopy at 5 K2011

    • Author(s)
      Uchiyama, Dewa (第5著者), Matsushita (他4名)
    • Journal Title

      Physical Chemistry Chemical Physics in press

    • Peer Reviewed
  • [Journal Article] Immobilization of Porphyrin Derivatives with a Defined Distance and Orientation onto a Gold Electrode Using Synthetic Light-Harvesting α-Helix Hydrophobic Polypeptides2010

    • Author(s)
      Ochiai, Dewa (第6著者) (他6名)
    • Journal Title

      Langmuir 26

      Pages: 14419-14422

    • Peer Reviewed
  • [Journal Article] Liposomal Polyamine-Dialkyl Phosphate Conjugates as Effective Gene Carriers : Chemical Structure, Morphology, and Gene Transfer activity2010

    • Author(s)
      Dewa (他11名)
    • Journal Title

      Bioconjugate Chem. 21

      Pages: 844-852

    • Peer Reviewed
  • [Journal Article] The Electronic behavior of a Photosynthetic Reaction Center Monitored by Conductive Atomic Force Microscopy2009

    • Author(s)
      Mikayama, Dewa (第4著者) (他6名)
    • Journal Title

      Journal of Nanoscience and Nanotechnology 9

      Pages: 97-107

    • Peer Reviewed
  • [Journal Article] How Deep Is the Potential Well Confining a Protein in a Specific Conformation? A Single-Molecule Study on Temperature Dependence of Conformational Change between 5 and 18 K2008

    • Author(s)
      Oikawa, Dewa (第3著者)(他3名)
    • Journal Title

      J.Am.Chem.Soc. 130

      Pages: 4580-4581

    • Peer Reviewed
  • [Presentation] 光合成膜タンパク質分子集合体の構築と機構評価2011

    • Author(s)
      出羽毅久, ら
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-28
  • [Presentation] Supramolecular Assembly of Bacterial Light-harvesting/reaction center complexes (LH2/LHl-RC) in Planar Lipid Bilayers : AFM Observation, Energy Transfer, and Photocurrent Generation.2010

    • Author(s)
      出羽毅久, et al.
    • Organizer
      The 70^<th> Okazaki Conference
    • Place of Presentation
      Okazaki
    • Year and Date
      2010-12-05
  • [Presentation] Functional Analysis of Poluamine-Lipid/DNA Complex (Lipoplex) as Gene Carrier : Relationship Between Metamorphosis of Lipoplex and its Function.2010

    • Author(s)
      Derma, et al.
    • Organizer
      The 4^<th> International Symposium : Molecular Science of Fluctuations toward Biological Functions.
    • Place of Presentation
      滋賀
    • Year and Date
      2010-11-30
  • [Presentation] 光合成膜タンパク質集合体の構築と機能解析2010

    • Author(s)
      出羽毅久, ら
    • Organizer
      第59回高分子討論会
    • Place of Presentation
      北海道大学(依頼講演)
    • Year and Date
      2010-09-15
  • [Presentation] Liposomal Polyamine-dialkyl Phosphate Conjugates as Effective Gene Carriers : Chemical Structure, Morphology, and Gene Transfer Activity.2010

    • Author(s)
      Dewa, et al.
    • Organizer
      International Liposome Research Days and Lipids, Liposomes & Membrane Biophysics
    • Place of Presentation
      Vancouver
    • Year and Date
      2010-08-06
  • [Presentation] Organization and AFM observation of photosynthetic membrane proteins assembly in planar lipid bilayer2010

    • Author(s)
      出羽毅久
    • Organizer
      13th International Conference on Non-Contact Atomic Force Microscopy, 167 Committee satellite workshop On SPM
    • Place of Presentation
      金沢(招待講演)
    • Year and Date
      2010-08-04
  • [Presentation] Organization and Functional Analysis of Supramolecular Assembly of Photosynthetic Membrane Proteins.2010

    • Author(s)
      出羽毅久, et al.
    • Organizer
      International Symposium on Chemical Conversion of Light Energy.
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-28
  • [Presentation] Morphological analysis of polyamine-lipid/DNA complexes as effective gene carriers.2009

    • Author(s)
      出羽毅久, et al.
    • Organizer
      The third international symposium on "molecular Science of Fluctuations toward Biological function.
    • Place of Presentation
      名古屋
    • Year and Date
      2009-12-20
  • [Presentation] AFM Observation of Photosynthetic Membrane Protein Assembly in Planar Lipid Bilayer.2009

    • Author(s)
      出羽毅久, et al.
    • Organizer
      Watching Biomolecules in Action (WBMA'09)-Single Molecule Biology Symposium
    • Place of Presentation
      大阪
    • Year and Date
      2009-12-16
  • [Presentation] 膜タンパク質研究のための平面脂質二分子膜の構築2009

    • Author(s)
      出羽毅久
    • Organizer
      第40回中部化学関係学協会支部連合秋季大会
    • Place of Presentation
      (岐阜大学)(依頼講演)
    • Year and Date
      2009-11-07
  • [Presentation] Liposomal Polyamine-Dialkyl Phosphate Conjugates as Effective Gene Carriers : Chemical Structure, Morphology, and Gene Transfer Activity.2009

    • Author(s)
      出羽毅久, et al.
    • Organizer
      International Symposium on Nanomedicine 2009-2
    • Place of Presentation
      岡崎
    • Year and Date
      2009-11-05
  • [Presentation] 平面脂質二分子膜中に導入した光合成アンテナ・反応中心複合体の原子間力顕微鏡による直接観察2008

    • Author(s)
      出羽毅久, ら
    • Organizer
      第3回バイオ関連化学.合同シンポジウム(東工人)
    • Year and Date
      2008-09-19
  • [Presentation] 形態観察を目的とした光合成アンテナ膜タンパク質の発現と複合体形成2008

    • Author(s)
      出羽毅久, ら
    • Organizer
      第18回バイオ・高分子シンポジウム
    • Place of Presentation
      上智大学
    • Year and Date
      2008-07-26
  • [Book] The Purple Photosynthetic Bacteria(Assembly of bacterial light harvesting complexes on solid substrates)2009

    • Author(s)
      K.Iida, T.Dewa, M.Nango
    • Total Pages
      861-875
    • Publisher
      Advances in Photosynthesis and Respiration
  • [Book] Molecular Assembly of Bacteriochlorophyll a Complexes Using Light-harvesting (LH) Polypeptide from Photosynthetic Bacteria and Its Model Synthetic Polypeptides(Bottom-up Nanofabrication : Supramolecules, Self-Assemblies, and Organized Films)(Chapter 6)2009

    • Author(s)
      M.Nango, M.Nagata, K.Iida, T.Dewa
    • Total Pages
      177-198
    • Publisher
      American Scientific Publishers (ASP)
  • [Remarks] ホームページ等

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Published: 2012-01-26   Modified: 2016-04-21  

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