2010 Fiscal Year Final Research Report
STRUCTUE ANALYSIS OF BIOMOLECULES BY SYNCHROTRON RADIATION VACUUM-ULTRAVIOLET CIRCULAR DICHROISM SPECTROSCOPY
| Project/Area Number |
20550153
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemistry related to living body
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
OHMAE Eiji Hiroshima University, 大学院・理学研究科, 助教 (30284152)
|
|---|
| Research Collaborator |
MATSUO Koichi 広島大学, 放射光科学研究センター, 特任助教 (40403620)
WOODY Robert W. 米国コロラド州立大学, 教授
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 放射光 / 真空紫外円二色性 / タンパク質 / 二次構造解析 / ニューラルネットワーク法 / 糖類 |
|---|
| Research Abstract |
Synchrotron-radiation vacuum-ultraviolet circular dichroism (VUVCD) spectroscopy was applied for the detailed structural analysis of biomolecules which was difficult by a conventional CD spectroscopy as follows : (1) prediction of amino acid sequence of protein secondary structures ; comprehensive secondary-structure analysis of disulfide variants of lysozyme ; (3) experimental and theoretical analyses of VUVCD spectra of mono- and polysaccharides ; (4) secondary structure analysis of acid-glycoprotein ; and (5) structural analysis of amyloid fibrils.
|
