2010 Fiscal Year Final Research Report
Functional analysis of Streptomyces prolyl oligopeptidase family
Project/Area Number |
20550158
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Chemistry related to living body
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Research Institution | Research Institute for Biological Science Okayama |
Principal Investigator |
HATANAKA Tadashi Research Institute for Biological Science Okayama, 酵素機能研究グループ, 研究員 (00344408)
|
Project Period (FY) |
2008 – 2010
|
Keywords | 酵素化学 / ペプチダーゼ / 放線菌 |
Research Abstract |
The aim of present study was to reveal enzymatic properties of three types belonged to a family of prolyl oligopeptidase in Streptomyces genomes. The evidence presented in this study demonstrates that one is an exo-aminopeptidase, and the other is an endo-peptidase. The later shows an trypsin-like activity, and is belonged to a family of oligopeptidase B. Furthermore, by substitution of the active serine residue in the aminopeptidase with cysteine, its aminolysis activity is more enhanced than that of the wild-type enzyme.
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Research Products
(16 results)