2010 Fiscal Year Final Research Report
Legionella in environmental water and determination of Legionella by a Novel DNA Pattern Analysis Method
| Project/Area Number |
20560502
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | University of Yamanashi |
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Principal Investigator |
HIRAYAMA Keiko University of Yamanashi, 大学院・医学工学総合研究部, 助教 (40111778)
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| Research Collaborator |
YANAGI Tooru (株)G&Gサイエンス基盤研究所, 所長
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| Project Period (FY) |
2008 – 2010
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| Keywords | 土壌 / 水環境 |
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| Research Abstract |
A survey of water samples in Yamanashi Prefecture, Japan, was conducted in order to determine the relationship between the growth of colony-forming Legionella and water quality parameters. Legionella was also analyzed by real time PCR (RT-PCR). Colony-forming Legionella was not detected in water samples in which phosphate ions were not measured. To control phosphate ion may be effective to protect from Legionella growth. RT-PCR Legionella was measured in all of the samples that were negative for colony-forming Legionella. The survival rates (colony-forming Legionella/RT-PCR Legionella × 100) were 0.1% to 0.5%. DNA pattern analysis of 15 species in the genus Legionella pneumophila was carried out by a novel DNA pattern analysis method (Genopattern method). DNA pattern of 15 species exhibits a specific pattern, which is different from other's. The result suggests determination by Genopattern method may be applicable to distinguish species of Legionella.
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