2010 Fiscal Year Final Research Report
Proteomic analysis of antimicrobial proteins from rice grain and clarification of their action mechanisms against periodontal pathogenic bacteria
| Project/Area Number |
20560725
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUCHI Masayuki Niigata University, 自然科学系, 教授 (00163634)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUBO Sadami 新潟県農業総合研究所, 食品研究センター, 専門研究員 (10503625)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 歯周病 / 抗菌ペプチド / 米タンパク質 / 食品素材 |
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| Research Abstract |
In this study, we found that the partial peptide CH 14-25 (RRLMAAKAESRK), identical with aa 14-25 of rice cyanate hydratase, has an antimicrobial activity against Porphyromonas gingivalis. We examined not only the contribution of cationic amino acid residues (R and K) of this peptide to the antimicrobial activity but also the action mechanisms of these CH peptides by the membrane depolarization assay and the microscopic observations of giant unilamellar vesicles. The results obtained indicate that the R24 and K25 residues are important for the CH peptides to exert their antimicrobial activity. The results obtained also indicate that the CH peptides depolarized the cytoplasmic membrane, resulting in the antimicrobial action. Our observations indicate that the CH peptides will be useful to develop preventive drugs and foods against periodontal disease.
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