2010 Fiscal Year Final Research Report
Fundamental process design for immobilized enzyme reaction in a gas/solid bioreactor
| Project/Area Number |
20560733
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kochi National College of Technology |
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Principal Investigator |
NAGAYAMA Kazuhito Kochi National College of Technology, 物質工学科, 准教授 (00270364)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 気相媒体 / 酵素反応 / ケトン還元酵素 / 立体選択性 / バイオリアクター |
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| Research Abstract |
The chiral alcohol production from ketone catalyzed by immobilized ketoreductases (alcohol dehydrogenases) has been investigated in a gas-solid bioreactor. As fundamental design of continuous reaction process, the conditions for immobilized enzyme preparation as functions of the reduced pressure degree, the buffer pH as well as the buffer concentration and for reaction as functions of the water concentration and the temperature were optimized. Also, the optimal reaction systems and enzyme sources for the gas phase reaction were investigated.
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