2010 Fiscal Year Final Research Report
| Project/Area Number |
20570002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
ONAI Kiyoshi Nagoya University, 遺伝子実験施設, 研究員 (00402454)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 生物時計 / 概日リズム / 生物発光 |
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| Research Abstract |
OsPCL1 is unique gene in the rice genome and homologous to the Arabidopsis clock gene PHYTOCLOCK1 (PCL1). The gene should be controlling the photoperiodic flowering in rice. We screened mutants containing mutations on OsPCL1 locus by TILLING. In ～3,600 EMS (ethylmethan sulfonate) mutated rice lines (M_3 or M_4), we isolated 7 OsPCL1 mutants. Three mutants had single nucleotide substitution in the coding sequence of OsPCL1 causing single amino acid substitution in OsPCL1 but showed little effects on the photoperiodic flowering and circadian gene expressions. Residual 4 mutants had silent mutation. These results suggest that more mutants are needed to clarify the gene functions. We have prepared OsPCL1 RNAi lines in rice and are investigating their phenotypes.
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[Journal Article] Plasma membrane aquaporin agpZ is essential for glucose metabolism during photomixotrophic growth of Synechocysiis sp. PCC 6803.2011
Author(s)
Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N
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Journal Title
Peer Reviewed
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[Journal Article] Functionally important structural elements of the cyanobacterial clock-related protein Pex.2009
Author(s)
Kurosawa S, Murakami R, Onai K(共著第一著者), Morishita M, Hasagawa D, Iwase R, Uzumaki T, Hyashi F,Kitajima-Ihara T, Sakata S, Mrakami M, Kouyama T, Ishiura M
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Journal Title
Genes Cells 14
Pages: 1-16
Peer Reviewed
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[Journal Article] Identification and characterization of the Na+/H+ antiportere NhaS3 from the thylakoid mambrane of Synechocystis sp.PCC 6803.2009
Author(s)
Tsunekawa K, Shijyuku T, Hayashimoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N
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Journal Title
J Biol Chem 284
Pages: 16513-16521
Peer Reviewed
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