Distribution of condensins and chromosome architecture

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570009
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ONO Takao The Institute of Physical and Chemical Research, 平野染色体ダイナミクス研究室, 専任研究員 (20291172)
• Project Period (FY): 2008 – 2010
• Keywords: コンデンシンI / コンデンシンII / 染色体凝縮 / 姉妹染色分体 / DNA複製 / 複製ストレス / 人工染色体 / ピストン修飾

Research Abstract

Eukaryotic cells possess two different condensin complexes, known as condensin I and II. Both play essential yet distinct functions in the processes of mitotic chromosome assembly and segregation. Interestingly, condensin II tends to be enriched in G-band regions. It has long been known that G-band regions are replicated later in S phase and condense earlier in prophase. This set of information allows us to propose the working hypothesis that condensin II may play a role in linking DNA replication in S phase to chromosome condensation in M phase. Quantitative cell imaging analyses combined with immunostaining show that condensin II start to associate with chromosomes during S phase. Interestingly, in the presence of replication stress, the depletion of condensin II, but not of condensin I, induced a critical abnormality in chromosome in M phase. These results suggest that condensin II may play a crucial role in structural conversion of replicated chromosomes during S phase to facilitate chromosome condensation in M phase.

Research Products

• [Journal Article] A novel mutation in the C-terminal region of RUNX2/CBFA1 distal to the DNA-binding runt domain in a Japanese patient with cleidocranial dysplasia. (2011)
  • Author(s): Kamamoto, M., Machida, J., Miyachi, H., Ono T., Nakayama, A., Shimozato, K., Tokita, Y
  • Journal Title: International Journal of Oral & Maxillofacial Surgery 40 Pages: 434-437
  • Peer Reviewed
• [Journal Article] Characterization of a de novo balanced t(4;20)(q33;ql2) translocation in a patient with mental retardation. (2010)
  • Author(s): Yamada, K., Fukushi, D., Ono T., Kondo, Y., Kimura, R., Nomura, N., Kosaki, KJ., Yamada, Y., Mizuno, S., Wakamatsu, N
  • Journal Title: American Journal of Medical Genetics, Part A 152A Pages: 3057-3067
  • Peer Reviewed
• [Journal Article] Searching for genes for cleft lip and/or palate based on breakpoint analysis of a balanced translocation t(9;17)(q32;q12). (2009)
  • Author(s): Machida, J., Felix, TM., Murray, JC., Yoshiura, K., Tanemura, M., Kamamoto, M., Shimozato, K., Sonta, S., Ono T.
  • Journal Title: Cleft Palate-Craniofacial Journal 46 Pages: 532-540
  • Peer Reviewed
• [Journal Article] 染色体の形作りとコンデンシンの役割 (2008)
  • Author(s): 小野教夫
  • Journal Title: 生体の科学(増大特集現代医学・生物学の仮説・学説2008) 59(5) Pages: 354-355
• [Presentation] 小頭症の原因タンパク質MCPH1によるコンデンシンIIの制御 (2010)
  • Author(s): 山下大輔、新冨圭史、小野教夫、平野達也
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Ki67抗原は分裂期染色体構築にどのように関与するのか (2010)
  • Author(s): 高木昌俊、西山祐子、小野教夫、平野達也、今本尚子
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] S期におけるコンデンシンIIの役割 (2010)
  • Author(s): 小野教夫、平野達也
  • Organizer: 染色体学会第61回年会
  • Place of Presentation: 船橋
  • Year and Date: 2010-11-07
• [Presentation] 染色体複製と凝縮の機能連係:コンデンシンIIの視点から (2009)
  • Author(s): 小野教夫、平野達也
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 2009-01-27
• [Presentation] Nuclear condensin II in S phase : linking DNA replication to chromosome condensation. (2008)
  • Author(s): Ono T., Hirano T.
  • Organizer: The 3rd Asian Chromosome Colloquium
  • Place of Presentation: 大阪
  • Year and Date: 2008-12-02
• [Remarks] ホームページ等
• [Remarks]
  • URL: http://www.riken.jp/index_j.html
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/wako/ch-dyna/index.html