Posttranscriptional regulation of plastid gene expression in Physcomitrella patens.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: SUGITA Mamoru Nagoya University, 遺伝子実験施設, 教授 (70154474)
• Project Period (FY): 2008 – 2010
• Keywords: 葉緑体 / ミトコンドリア / 転写後制御 / RNA編集 / PPRタンパク質 / ヒメツリガネゴケ / 遺伝子破壊株

Research Abstract

Chloroplasts possess their own DNA and approximately 100 genes. Chloroplast function and gene expression are cooperatively controlled by the chloroplast and nuclear genomes. Posttranscriptional regulation is an important step in the control of chloroplast gene expression. In this study, we identified novel nuclear-encoded pentatricopeptide repeat proteins involved in RNA splicing, RNA cleavage, and RNA splicing in chloroplasts.

Research Products

• [Journal Article] The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants. (2011)
  • Author(s): Banks, J.A., Nishiyama, T., Hasebe, M., Sugita, M.
  • Journal Title: Science 332(6032) Pages: 960-963(DOI: 10.1126/Science.1203810)
• [Journal Article] DNA packaging proteins Glom and Glom2 coordinately organize the mitochondrial nucleoid of Physarum polycephalum. (2011)
  • Author(s): Itoh, K., Izumi, A., Mori, T., Dohmae, N., Yui, R., Maeda-Sano, K., Shirai, Y., Kanaoka, M.M., Kuroiwa, T., Higashiyama, T., Sugita, M., Murakami-Murofushi, K., Kawano, S., Sasaki, N.
  • Journal Title: Mitochondrion in press Pages: Doi: 10.1016/j/mito
• [Journal Article] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts. (2010)
  • Author(s): Ohtani, S., Ichinose, M., Tasaki, E., Aoki, Y., Komura, Y., Sugita, M.
  • Journal Title: Plant and Cell Physiology 52(11) Pages: 1942-1949
• [Journal Article] The moss Physcomitrella patens, a model plant for the study of RNA editing in plant organelles. (2010)
  • Author(s): Tasaki, E., Sugita, M.
  • Journal Title: Plant Signaling & Behavior 5(6) Pages: 727-729
• [Journal Article] The moss pentatricopeptide repeat protein with a DYW domain is responsible for RNA editing of mitochondrial ccmFc transcript. (2010)
  • Author(s): Tasaki, E., Hattori, M., Sugita, M.
  • Journal Title: The Plant Journal 62(4) Pages: 560-570
• [Journal Article] A moss pentatricopeptide repeat protein binds to the 3'-end of plastid clpP pre-mRNA and assists with the mRNA maturation. (2009)
  • Author(s): Hattori, M., Sugita, M.
  • Journal Title: FEBS Journal 276(20) Pages: 5860-5869
• [Journal Article] Cyanobacterial daily life with Kai-based circadian and diurnal genome-wide transcriptional control in Synechococcus elongatus. (2009)
  • Author(s): Ito, H., Mutsuda, M., Murayama, Y., Tomita, J., Hosokawa, N., Terauchi, K., Sugita, C., Sugita, M., Kondo, T., Iwasaki, H.
  • Journal Title: Proc.Natl Acad.Sci.USA 106(33) Pages: 14168-14173
• [Journal Article] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. (2009)
  • Author(s): Okuda, K.Chateigner-Boutin, A.L., Nakamura, T., Delannoy, E., Sugita, M., Myouga, F., Motohashi, R., Shinozaki, K., Small, I.Shikanai, T.
  • Journal Title: The Plant Cell 21 Pages: 146-156
• [Journal Article] A conserved DYW domain of the pentatricopeptide repeat protein possesses a novel endoribonuclease activity. (2008)
  • Author(s): Nakamura, T., Sugita, M.
  • Journal Title: FEBS Letters 582(30) Pages: 4163-4168
• [Journal Article] Characteristics and prediction of RNA editing sites in the transcripts of a moss Takakia lepidozioides chloroplast. (2008)
  • Author(s): Yura, K., Miyata, Y., Arikawa, T., Higuchi, M., Sugita, M.
  • Journal Title: DNA Research 15(5) Pages: 309-321
• [Journal Article] Suppression mechanism of mitochondrial ORF79 accumulation by Rf1 protein in BT-type cytoplasmic male sterile rice. (2008)
  • Author(s): Kazama, T., Nakamura, T., Watanabe, M.,Sugita, M., Toriyama, K.
  • Journal Title: The Plant Journal 55 Pages: 619-628
• [Journal Article] On the expansion of the pentatricopeptide repeat gene family in plants. (2008)
  • Author(s): O'Toole, N., Hattori, M., Andres, C., Iida, K., Claire Lurin, C., Schmitz-Linneweber, C., Sugita, M., Small, I.
  • Journal Title: Molecular Biology and Evolution 25(6) Pages: 1120-1128
• [Journal Article] RNA editing in the anticodon of tRNALeu (CAA) occurs before group I intron splicing in plastids of a moss Takakia lepidozioides S. Hatt. & Inoue. (2008)
  • Author(s): Miyata, Y., Sugita, C., Maruyama, K., Sugita, M.
  • Journal Title: Plant Biology 10 Pages: 250-255
• [Presentation] Physcomitrella patens, a model plant for studying the molecular basis of site-specific RNA editing (T30). (2010)
  • Author(s): Mamoru Sugita, Shotaro Ohtani, Mizuho Ichinose, Masato Uchida, Chieko Sugita
  • Organizer: Oral presented on July 24.MOSS2010, the annual international conference for experimental moss research
  • Place of Presentation: in Sapporo and its vicinity, Lake Shikotsu Resort,Hokkaido, Japan.
  • Year and Date: 20100721-20100724
• [Presentation] DYWドメインを持つPPRタンパク質はミトコンドリアmRNAの蓄積レベルを制御する(2pG03) (2010)
  • Author(s): 一瀬瑞穂、田崎瑛示、杉田護
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2010-09-10
• [Presentation] ヒメツリガネゴケのPPRタンパク質は質は部位特異的なRNA編集やRNAの蓄積レベルの制御に働く(3pH02) (2010)
  • Author(s): 田崎瑛示、香村吉洋、青木良晃、大谷祥太郎、永島はるか、杉田護
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] RNA結合性pentatricopeptide repeat(PPR)タンパク質ファミリーの網羅的解析(4P-301) (2009)
  • Author(s): 杉田護、田崎瑛示、香村吉洋、青木良晃、井手瑞樹、服部満
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Helical repeat proteinのRNA配列特異性と生体プローブへの応用(1P-0881) (2008)
  • Author(s): 服部満、杉田護、小澤岳昌
  • Organizer: 第31回日本分子生物学会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 陸上植物の葉緑体RNA編集の進化 (2008)
  • Author(s): 宮田有希、杉田護
  • Organizer: 日本植物学会第72回大会(高知)
  • Place of Presentation: 高知大学
  • Year and Date: 2008-09-25
• [Book] Chapter 8.Chloroplasts.In The Moss Physcomitrella patens(Celia Knight, Pierre Francois-Perround and David Cove, eds.)(Annual Plant Reviews 36) (2009)
  • Author(s): Sugita, M., Aoki, S.
  • Total Pages: 182-210
  • Publisher: Blackwell Publishing
• [Book] 5章形質転換(4.ヒメツリガネゴケ、低温科学67巻) (2009)
  • Author(s): 杉田護、田崎英示、香村吉洋
  • Total Pages: 607-613
  • Publisher: 北海道大学低温科学研究所
• [Remarks] ホームページ等
  • URL: http://www.gene.nagoya-u.ac.jp/?sugita-g/
• [Remarks]
  • URL: http://www.bio.nagoya-u.ac.jp/index2.html