Neuroendocrine basis of lunar-synchronized spawning migration in grass puffer

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Kyushu University
• Principal Investigator: ANDO Hironori Kyushu University, 大学院・農学研究院, 准教授 (60221743)
• Co-Investigator(Kenkyū-buntansha): HATTORI Atsuhiko 東京医科歯科大学, 教養部, 教授 (70183910)
• Project Period (FY): 2008 – 2010
• Keywords: 産卵回遊 / 生殖リズム / 松果体 / メラトニン / GnRH / キスペプチン / GnIH

Research Abstract

To establish molecular basis of neuroendocrine control of lunar-synchronized spawning migration in grass puffer, we assessed changes in magnitudes of gene expression for various neurohormones and their receptors in the hypothalamus and pineal gland. In the hypothalamus, the expressions of those genes significantly increased during the spawning period and showed diurnal and circadian variations in association with changes in expression of melatonin receptor gene. In the pineal gland, melatonin receptor gene showed unique rhythmic expressions with 14 hours cyclicity and monthly variations. Observations of spawning behavior in spawning grounds and in aquarium revealed that the spawning rhythm is endogenously maintained possibly under the control of the circalunar clock in the pineal gland.

Research Products

• [Journal Article] Synchronised expressions of LPXRFamide peptide and its receptor genes : seasonal, diurnal and circadian changes during spawning period in grass puffer. (2010)
  • Author(s): Shahjahan, Md., Ikegami, T., Osugi, T., Ukena,K., Doi,H., Hattori, A., Tsutsui, K., Ando, H.
  • Journal Title: J. Neuroendocrinol. 23 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Elevation of Kiss2 and its receptor gene expression in the brain and pituitary of grass puffer during the spawning season. (2010)
  • Author(s): Shahjahan, Md., Motohashi, E., Doi, H., Ando, H.
  • Journal Title: Gen. Comp. Endocrinol. 169 Pages: 48-57
  • Peer Reviewed
• [Journal Article] Differential expression of three types of gonadotropin-releasing hormone genes during the spawning season in grass puffer, Takifugu niphobles. (2010)
  • Author(s): Shahjahan, Md., Hamabata, T., Motohashi, E., Doi, H., Ando, H.
  • Journal Title: Gen. Comp. Endocrinol. 167 Pages: 153-163
  • Peer Reviewed
• [Journal Article] Aggregating behavior of grass puffer, Takifugu niphobles, observed in aquarium during the spawning period. (2010)
  • Author(s): Motohashi, E., Yoshihara, T., Doi, H., Ando, H.
  • Journal Title: Zool. Sci. 27 Pages: 559-564
  • Peer Reviewed
• [Journal Article] Melatonin stimulates the release of gonadotropin-inhibitory hormone by the avian hypothalamus. (2010)
  • Author(s): Chowdhury, V.S., Yamamoto, K., Ubuka, T., Bentley, G.E., Hattori, A., Tsutsui, K.
  • Journal Title: Endocrinology 151 Pages: 271-280
  • Peer Reviewed
• [Journal Article] Synchronized diurnal and circadian expressions of four subtypes of melatonin receptor genes in the diencephlon of a puffer fish with lunar-related spawning cycles. (2009)
  • Author(s): Ikegami, T., Motohashi, E., Doi, H., Hattori, A., Ando, H.
  • Journal Title: Neurosci. Lett. 462 Pages: 58-63
  • Peer Reviewed
• [Journal Article] Diurnal expressions of four subtypes of melatonin receptor genes in the optic tectum and retina of goldfish. (2009)
  • Author(s): Ikegami, T., Azuma, K., Nakamura M., Suzuki, N., Hattori, A., Ando, H.
  • Journal Title: Comp. Biochem. Physiol. Part A 152 Pages: 219-224
  • Peer Reviewed
• [Journal Article] Structure of neurohypophysial hormone genes and changes in the levels of expression during spawning season in grass puffer (Takifugu niphobles). (2008)
  • Author(s): Motohashi, E., Hamabata, H., Ando, H.
  • Journal Title: Gen. Comp. Endocrinol. 155 Pages: 456-463
  • Peer Reviewed
• [Journal Article] Mini Review : Novel bromomelatonin derivatives as potentially effective drugs to treat bone diseases. (2008)
  • Author(s): Suzuki, N., Somei, M., Seki, A., Reiter, R.J., Hattori, A.
  • Journal Title: J. Pineal Res. 45 Pages: 229-234
  • Peer Reviewed
• [Presentation] Neuroendocrine regulation of spawning migration in grass puffer. (2010)
  • Author(s): Ando, H.
  • Organizer: 中国農業大学生物学院特別セミナー
  • Place of Presentation: 北京
  • Year and Date: 20100800
• [Presentation] Neuroendocrine mechanisms of spawning migration insalmonids. (2010)
  • Author(s): Ando, H., Urano, A.
  • Organizer: 9^<th> International Congress of the Biology of Fish
  • Place of Presentation: バルセロナ
  • Year and Date: 20100700
• [Presentation] メラトニンの効果と各種食材に含まれるメラトニン (2010)
  • Author(s): 服部淳彦
  • Organizer: 日本抗加齢医学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100600
• [Presentation] Neuroendocrine regulation of spawning behavior in grass puffer, Takifugu niphobles. (2009)
  • Author(s): Ando, H.
  • Organizer: 中国動物学会比較内分泌学分会第八回学術会議
  • Place of Presentation: 麗江
  • Year and Date: 20090900
• [Presentation] Melatonin and Anti-aging. (2009)
  • Author(s): 服部淳彦
  • Organizer: YIES Symposium "Biorhythms and Health"
  • Place of Presentation: 山梨
  • Year and Date: 20090700
• [Book] 身近な動物を使った実験1(第3章キンギョ)(鈴木範男編) (2009)
  • Author(s): 鈴木信雄, 田畑純, 服部淳彦
  • Total Pages: 46
  • Publisher: 三共出版
• [Remarks] ホームページ等
  • URL: http://www.sc.niigata-u.ac.jp/sc/sadomarine/
• [Patent(Industrial Property Rights)] Tryptophan derivative and application thereof. (2010)
  • Inventor(s): 染井正徳、服部淳彦、鈴木信雄
  • Industrial Property Rights Holder: 染井正徳、服部淳彦、鈴木信雄
  • Industrial Property Number: Patent,US 7,659,304 B2
  • Filing Date: 20100200
  • Overseas