2010 Fiscal Year Final Research Report
Immunocytochemical studies on the behavior of LHCP II in wax-rich cells of Euglena.
| Project/Area Number |
20570062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Nippon Sport Science University |
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Principal Investigator |
OSAFUNE Tetsuaki Nippon Sport Science University, 体育学部, 教授 (70074630)
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| Co-Investigator(Kenkyū-buntansha) |
EHARA Tomoko 東京医科大学, 医学部, 助手 (40468658)
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| Project Period (FY) |
2008 – 2010
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| Keywords | Euglena / immunoelectron microscopy / LHCP II / Golgi body / plastid / Golgi |
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| Research Abstract |
Immunoelectron microscopy of wax-rich cells of Euglena gracilis Z using anti-LHCP II antibody and protein A-gold shows LHCP II first in the Cos, Golgi body and Golgi-derived vesicle by the thylakoids of the plastid. Golgi-derived vesicle fuses with the outermost membrane of the Euglena plastid. In the presence of 0.1% Streptomycin have plastids in which the disappearance of the prolamellar body (PLB) is inhibited ; a paracrystalline body is formed in close proximity to the the PLB and pyrenoid which shows an immunoreaction with LHCP II antibody, but the immunoreaction is absent from the thylakoids. Thus conditions in the aerated wax-rich cells allow an unusual accumulation of LHCP II apoprotein at Streptomycin blocks the distribution of this apoprotein to the thylakoids.
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