Analysis of molecular evolution of sex steroid hormone receptors

2010 Fiscal Year Final Research Report

• Project/Area Number: 20570064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: KATSU Yoshinao National Institutes of Natural Sciences Okazaki Research Facilities, 大学院・理学研究院, 准教授 (00332180)
• Project Period (FY): 2008 – 2010
• Keywords: 比較内分泌 / 分子進化 / ホルモン受容体

Research Abstract

Estrogens are necessary for ovarian differentiation in most vertebrates including human. Estrogen actions are largely mediated through the estrogen receptors (ERs), which are hormone activated transcription factors. To understand the molecular evolution of ER, we tried to isolate cDNAs encoding ERs from amphioxus, ancient fishes, and other species. We found that amphioxus that is an invertebrate has already possessed the ER gene. Our functional analyses suggest that the function of ER as a hormone-dependent transcription factor might be gained through the evolutionary process.

Research Products

• [Journal Article] Estrogen-dependent transactivation of amphioxus steroid hormone receptor via both estrogen- and androgen-response elements. (2010)
  • Author(s): Katsu Y, Kubokawa K, Urushitani H, Iguchi T.
  • Journal Title: Endocrinology 151 Pages: 639-648
  • Peer Reviewed
• [Journal Article] Molecular cloning, characterization and chromosome mapping of reptilian estrogen receptors. (2010)
  • Author(s): Katsu Y, Matsubara K, Kohno S, Matsuda Y, Toriba M, Oka K, Guillette LJ, Ohta Y, Iguchi T.
  • Journal Title: Endocrinology 151 Pages: 5710-5720
  • Peer Reviewed
• [Journal Article] Cloning and functional characterization of Chondrichtyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors. (2010)
  • Author(s): Katsu Y, Kohno S, Narita H, Urushitani H, Yamane K, Hara A, Clauss TM, Walsh MT, Miyagawa S, Guillette LJ, Iguchi T.
  • Journal Title: Gen.Comp.Endocrinol. 168 Pages: 496-504
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of ligand- and species-specificity of amphibian estrogen receptors. (2010)
  • Author(s): Katsu Y, Taniguchi E, Urushitani H, Miyagawa S, Takase M, Kubokawa K, TooiO, Oka T, Santo N, Myburgh J, Matsuno A, Iguchi T.
  • Journal Title: Gen.Comp.Endocrinol. 168 Pages: 220-230
  • Peer Reviewed
• [Journal Article] Transcriptional activity and biological effects of mammalian estrogen receptor ligands on three hepatic estrogen receptors in Mozambique tilapia. (2010)
  • Author(s): Davis L, Katsu Y, Iguchi T, Lerner DT, Hirano T, Grau EG.
  • Journal Title: J.Steroid Biochem.Mol.Biol. 122 Pages: 272-278
  • Peer Reviewed
• [Journal Article] Influences of sex, incubation temperature, and environmental quality on gonadal estrogen and androgen receptor messenger RNA expression in juvenile American alligator (Alligator mississippiensis). (2010)
  • Author(s): Moore BC, Milnes MR, Kohno S, Katsu Y, Iguchi T, Guillette LJ.
  • Journal Title: Biol.Reprod. 82 Pages: 194-201
  • Peer Reviewed
• [Journal Article] Estrogen receptor ESR1 is indispensable for the induction of persistent vaginal change by neonatal 5alpha-dihydrotestosterone exposure in mice. (2010)
  • Author(s): Miyagawa S, Katsu Y, Ohta Y, Sudo T, Lubahn DB, Iguchi T.
  • Journal Title: Biol.Reprod. 82 Pages: 497-503
  • Peer Reviewed
• [Journal Article] Sexual reprogramming and estrogenic sensitization in wild fish exposed to ethinylestradiol. (2009)
  • Author(s): Lange A, Paull GC, Coe TS, Katsu Y, Urushitani H, Iguchi T, Tyler CR.
  • Journal Title: Environ.Sci.Technol. 43 Pages: 1219-1225
  • Peer Reviewed
• [Journal Article] Environmental health impacts of equine estrogens derived from hormone replacement therapy. (2009)
  • Author(s): Tyler CR, Filby AL, Bickley LK, Cumming RI, Gibson R, Labadie P, Katsu Y, Liney KE, Shears JA, Silva-Castro V, Urushitani H, Lange A, Winter MJ, Iguchi T, Hill EM.
  • Journal Title: Environ.Sci.Technol. 43 Pages: 3897-3904
  • Peer Reviewed
• [Journal Article] From reptilian phylogenomics to reptilian genomes : analyses of c-Jun and DJ-1 proto-oncogenes. (2009)
  • Author(s): Katsu Y*, Braun EL*, Guillette LJ, Iguchi T.
  • Journal Title: Cyto.Gen.Res. 127 Pages: 79-93
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of estrogen, androgen and progesterone nuclear receptors from a fresh turtle (Pseudemys nelsoni). (2008)
  • Author(s): Katsu Y, Ichikawa R, Ikeuchi T, Kohno S, Guillette LJ, Iguchi T.
  • Journal Title: Endocrinology 149 Pages: 161-173
  • Peer Reviewed
• [Journal Article] Increased post hatching mortality and loss of sexually dimorphic gene expression in alligator (Alligator mississippiensis) from a contaminated environment. (2008)
  • Author(s): Milnes MR, Bryan TA, Katsu Y, Kohno S, Moore BC, Iguchi T, Guillette LJ.
  • Journal Title: Biol.Reprod. 78 Pages: 932-938
  • Peer Reviewed
• [Journal Article] Gene expression patterns in juvenile American alligator (Alligator mississippiensis) exposed to environmental contaminants. (2008)
  • Author(s): Kohno S, Bermudez D, Katsu Y, Iguchi T, Guillette LJ.
  • Journal Title: Aqua.Toxicol. 88 Pages: 95-101
  • Peer Reviewed
• [Journal Article] Activation of steroid and xenobioticreceptor (SXR, NR112) and its orthologs in laboratory, toxicological, and genome model species. (2008)
  • Author(s): Milnes MR, Garcia A, Grossman E, Grun F, Shiotsugu J, Tabb MM, Kawashima Y, Katsu Y, Watanabe H, Iguchi T, Blumberg B.
  • Journal Title: Environ.Health Perspect. 116 Pages: 880-885
  • Peer Reviewed
• [Journal Article] Altered sexual development in roach (Rutilus rutilus) exposed to environmental concentrations of the pharmaceutical 17?-ethinylestradiol and associated expression dynamics of aromatases and estrogen receptors. (2008)
  • Author(s): Lange A, Katsu Y, Ichikawa R, Paull GC, Chidgey LL, Coe TS, Iguchi T, Tyler CR.
  • Journal Title: Toxicol.Sci. 106 Pages: 113-123
  • Peer Reviewed
• [Journal Article] Molecular cloning, characterization and evolutionary analysis of estrogen receptors from phylogenetically ancient fish. (2008)
  • Author(s): Katsu Y, Kohno S, Hyodo S, Ijiri S, Adachi S, Hara A, Guillette LJ, Iguchi T.
  • Journal Title: Endocrinology 149 Pages: 6300-6310
  • Peer Reviewed
• [Journal Article] The influence of non-toxic concentrations of DDT and DDE on the old world vulture estrogen receptor alpha. Gen. Comp. (2008)
  • Author(s): Naidoo V, Katsu Y, Iguchi T.
  • Journal Title: Endocrinol 159 Pages: 188-195
  • Peer Reviewed
• [Journal Article] Estrogen receptor subtypes selectively mediate female mouse reproductive abnormalities induced by neonatal exposure to estrogenic chemicals. (2008)
  • Author(s): Nakamura T, Katsu Y, Watanabe H, Iguchi T.
  • Journal Title: Toxicology 253 Pages: 117-124
  • Peer Reviewed
• [Journal Article] Novel approaches for the study of vertebrate steroid hormone receptors. (2008)
  • Author(s): Kohno S, Katsu Y, Iguchi T, Guillette LJ.
  • Journal Title: Integ.Comp.Biol. 48 Pages: 527-534
  • Peer Reviewed
• [Journal Article] Commonality and difference in mechanisms of endocrine disruption in various animal species. (2008)
  • Author(s): Iguchi T, Katsu Y.
  • Journal Title: BioScience 58 Pages: 1061-1067
  • Peer Reviewed
• [Presentation] 無顎類ヤツメウナギのエストロゲン受容体の単離 (2010)
  • Author(s): 勝義直・井口泰泉
  • Organizer: 日本動物学会第81回東京大会
  • Year and Date: 2010-09-23
• [Presentation] ワニのコルチコイド受容体遺伝子の単離と機能解析 (2010)
  • Author(s): 岡香織・勝義直
  • Organizer: 日本動物学会北海道支部大会
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] 軟骨魚類のエストロゲン受容体遺伝子単離と機能解析 (2010)
  • Author(s): 成田晴香・勝義直
  • Organizer: 日本動物学会北海道支部大会
  • Place of Presentation: 旭川
  • Year and Date: 2010-08-21
• [Presentation] ナメクジウオの2種類のステロイドホルモン受容体遺伝子の単離と機能解析 (2009)
  • Author(s): 勝義直・窪川かおる・漆谷博志・井口泰泉
  • Organizer: 日本動物学会第80回静岡大会
  • Year and Date: 2009-09-19
• [Presentation] Comparative responsiveness and sensitivity of fish oestrogen receptors to steroid oestrogens and environmental oestrogens. (2008)
  • Author(s): Katsu Y, Lange A, Tyler CR, Iguchi T
  • Organizer: The 10^<th> UK-Japan Annual Scientific Workshop
  • Place of Presentation: Devon, UK
  • Year and Date: 2008-10-06
• [Presentation] 古代魚のエストロゲン受容体の単離 (2008)
  • Author(s): 勝義直・井口泰泉
  • Organizer: 日本動物学会第79回福岡大会
  • Year and Date: 2008-09-05
• [Presentation] Molecular cloning, characterization and evolutionary analysis of estrogen receptor from phylogenetically ancient fish. (2008)
  • Author(s): 勝義直
  • Organizer: Summer Program in University of Hawaii
  • Year and Date: 2008-08-22
• [Book] In : Toxicogenomics : A powerful Tool for Toxicology Assessment.(Toxicogenomics and ecotoxicogenomics : Studying chemical effects and basic biology in vertebrates and invertebrates.) (2008)
  • Author(s): Iguchi T, Watanabe H, Katsu Y.
  • Publisher: John Wiley & Sons.
• [Remarks] ホームページ等
  • URL: http://www.repdev-katsu.jp/