Structure, function and evolution of arginine kinase from Tetrahymena piriformis

2011 Fiscal Year Final Research Report

• Project/Area Number: 20570072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Kochi University
• Principal Investigator: SUZUKI Tomohiko 高知大学, 教育研究部・自然科学系, 教授 (60145109)
• Co-Investigator(Kenkyū-buntansha): UDA Kouji 高知大学, 教育研究部・自然科学系, 助教 (10448392)
• Project Period (FY): 2008 – 2011
• Keywords: 代謝生理

Research Abstract

Two arginine kinases(AK1 and AK2) of Tetrahymena piriformis were characterized based on their structural and enzymatic properties. The AK2 had an unusual two-domain structure with two actives sites in a molecule. Intracellular localization of the two AKs was examined by using Western blotting and immunofluorescence technique, and the AK1 was identified unambiguously to localizing in cilia. On the other hand, AK2 appears to be distributed widely in cell body. We suggest that the localization of AK1 in cilia is promoted by putative myristoylated group of AK1.

Research Products

• [Journal Article] A diverse array of creatine kinase and arginine kinase isoform genes is present in the starlet sea anemone Nematostella vectensis, a cnidarian model system for studying developmental evolution (2012)
  • Author(s): Uda, K., Ellington, W. R., Suzuki, T
  • Journal Title: Gene Volume: 497 Pages: 214-227
  • Peer Reviewed
• [Journal Article] Cold-adapted features of arginine kinase from the deep-sea clam Calyptogena kaikoi (2012)
  • Author(s): Suzuki, T., Yamamoto, K., Tada, H. and Uda, K
  • Journal Title: Marine Biotechnology Volume: 14 Pages: 294-303
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues responsible for taurocyamine binding in mitochondrial taurocyamine kinase from Arenicola brasiliensis (2011)
  • Author(s): Tanaka, K., Matsumoto, T. and Suzuki T
  • Journal Title: Biochim. Biophys. Acta Volume: 1814 Pages: 1219-1225
  • Peer Reviewed
• [Journal Article] Arginine kinase from the tardigrade Macrobiotus occidentalis. Molecular cloning, phylogenetic analysis and enzymatic properties (2010)
  • Author(s): Uda, K., Ishida, M., Matsui, T. and Suzuki, T
  • Journal Title: Zool. Sci Volume: 27 Pages: 796-803
  • Peer Reviewed
• [Journal Article] Cooperativity in the two-domain arginine kinase from the sea anemone Anthopleura japonicus. II. Evidence from site-directed mutagenesis studies (2010)
  • Author(s): Tada, H. and Suzuki, T
  • Journal Title: Int. J. Biol. Macromol Volume: 47 Pages: 250-254
  • Peer Reviewed
• [Journal Article] Identification of key amino acid residues distinguishing chiral guanidino substrates(D-and L-arginine) in Sabellastarte arginine kinase (2010)
  • Author(s): Uda, K., Matumoto, A., and Suzuki, T
  • Journal Title: J. Molecular Catalysis Volume: 64 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Structural basis for the mechanism and substrate specificity of glycocyamine kinase, a phosphagen kinase family member (2010)
  • Author(s): Lim, K., Pullalarevu, S., Surabian, K. T., Howard, A., Suzuki, T., Moult, J., and Herzberg, O
  • Journal Title: Biochemistry Volume: 49 Pages: 2031-2041
  • Peer Reviewed
• [Journal Article] Molecular characterization and kinetic properties of a novel two-domain taurocyamine kinase from the lung fluke Paragonimus westermani (2009)
  • Author(s): Jarilla, B. R., Tokuhiro, S., Nagataki, M., Hong, S-J., Uda, K., Suzuki, T., and Agatsuma, T
  • Journal Title: FEBS Lett Volume: 583 Pages: 2218-2224
  • Peer Reviewed
• [Journal Article] The Role of Arg-96 in Danio rerio Creatine Kinase in Substrate Recognition and Active Center Configuration (2009)
  • Author(s): Uda, K., Kuwasaki, A., Shima, K., Matsumoto, T. and Suzuki, T
  • Journal Title: Int. J. Biol. Macromol Volume: 44 Pages: 413-418
  • Peer Reviewed
• [Journal Article] A novel arginine kinase from the shrimp Neocaridina denticulata : the fourth arginine kinase gene lineage (2009)
  • Author(s): Iwanami, K., Iseno, S., Uda, K. and Suzuki, T
  • Journal Title: Gene Volume: 437 Pages: 80-87
  • Peer Reviewed
• [Journal Article] Evolution of the diverse array of phosphagen systems present in annelids (2009)
  • Author(s): Suzuki, T., Uda, K., Adachi, M., Sanada, H., Tanaka, K., Mizuta, C., Ishida, K., and Ellington, W. R
  • Journal Title: Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol Volume: 152 Pages: 60-66
  • Peer Reviewed
• [Journal Article] Two-domain arginine kinase from the deep-sea clam Calyptogena kaikoi-Evidence of two active domains (2008)
  • Author(s): Uda, K., Yamamoto, K., Iwasaki, I., Iwai, M., Fujikura, K., Ellington, W. R., and Suzuki, T
  • Journal Title: Comp. Biochem. Physiol. Part B. Biochem. Mol. Biol Volume: 151 Pages: 176-182
  • Peer Reviewed
• [Journal Article] Cooperativity in the Two-domain Arginine Kinase from the Sea Anemone Anthopleura japonicus (2008)
  • Author(s): Tada, H., Nishimura, Y., and Suzuki, T
  • Journal Title: Int. J. Biol. Macromol Volume: 42 Pages: 46-51
  • Peer Reviewed
• [Presentation] テトラヒメナ(Tetrahymena pyriformis)のアルギニンキナーゼ(AK)のN-ミリストイル化 (2011)
  • Author(s): 岡崎紀子,宇田幸司,鈴木知彦
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-22
• [Presentation] テトラヒメナ(Tetrahymena pyriformis)アルギニンキナーゼの酵素機能解析 (2010)
  • Author(s): 岡崎紀子,道端珠梨,桜木陽子,宇田幸司,鈴木知彦
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-25
• [Presentation] テトラヒメナの2種類のアルギニンキナーゼの局在と役割 (2010)
  • Author(s): 道端珠梨,岡崎紀子,宇田幸司,鈴木知彦
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-23
• [Presentation] テトラヒメナの2種類のアルギニンキナーゼの性質と局在I (2009)
  • Author(s): 道端珠梨,宇田幸司,鈴木知彦
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-20
• [Presentation] テトラヒメナのアルギニンキナーゼの構造,機能及び進化 (2008)
  • Author(s): 松本保,岡添七香,桜木陽子,宇田幸司,鈴木知彦
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-07
• [Remarks]
  • URL: (http://p164056.cc.kochi-u.ac.jp/)