2010 Fiscal Year Final Research Report
DAG lipase-lipoxygenase pathway in Ca sensing of parathyroid cells
| Project/Area Number |
20570073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Nagasaki University |
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Principal Investigator |
OKADA Yukio Nagasaki University, 大学院・医歯薬学研究科, 准教授 (60136687)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIYAMA Rie 長崎大学, 大学院・医歯薬学研究科, 助教 (10274664)
HOTKEZAKA Hitoshi 長崎大学, 大学院・医歯薬学研究科, 講師 (90199513)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 動物生理化学 / 細胞シグナル伝達 |
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| Research Abstract |
Elevation of extracellular Ca^<2+> concentration induces intracellular Ca^<2+> signaling in parathyroid cells. We report the electrophysiological property associated with intracellular Ca^<2+> signaling in frog parathyroid cells and show that Ca^<2+>-activated Cl- channels are activated by intracellular Ca^<2+> increase through inositol 1,4,5-trisphophate (IP3)-independent pathway. High extracellular Ca^<2+> induced an outwardly-rectifying conductance in a dose-dependent manner. The conductance was composed of an instantaneous time-independent component and a slowly activating time-dependent component and displayed deactivating inward tail current. Extracellular Ca^<2+>-induced and Ca^<2+> dialysis-induced currents reversed at the equilibrium potential of Cl- and were inhibited by niflumic acid. Extracellular Ca^<2+>-induced currents displayed a moderate dependency on guanosine triphosphate (GTP). All blockers for phospholipase C, DAG lipase, MAG lipase and lipoxygenase inhibited extracellular Ca^<2+>-induced current. IP3 dialysis failed to induce conductance increase, but 2-AG, arachidonic acid and 12(S)-HPETE dialysis increased the conductance identical to extracellular Ca^<2+>-induced conductance. These results indicate high extracellular Ca^<2+> raises intracellular Ca^<2+> concentration through DAG lipase/lipoxygenase pathway, resulting in the activation of Cl^-conductance.
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[Presentation] 味覚障害におけるアイスキューブ刺激の効果
Author(s)
藤山理恵, 石飛進吾, 本多啓子, 前田香代子, 濱口盛子, 岡田幸雄, 大井久美子, 戸田一雄
Organizer
第14回日本摂食・嚥下リハビリテーション学会学術大会
Place of Presentation
千葉
Year and Date
00000900
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