2010 Fiscal Year Final Research Report
Structural change of telomere DNA induced by radical reaction
| Project/Area Number |
20570108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
KANAORI Kenji Kyoto Institute of Technology, 工芸科学研究科, 准教授 (30273543)
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| Research Collaborator |
TAJIMA Kunihiko 京都工芸繊維大学, 工芸科学研究科, 教授 (50163457)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ラジカル / 核酸 / 損傷 / NMR / ESR |
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| Research Abstract |
Telomere that exists in eukaryote's chromosome terminus is composed of telomeric DNA with the repetitive sequence to which the guanine base is abundant and of telomere binding proteins. The damage of the telomere sequence was verified by researching this telomeric site by using stopped-flow-ESR and NMR and generating superoxide anion radical and singlet oxygen. In addition, telomere DNA damage with the nitrous acid was concurrently studied, and the cross-linking reaction with the protein was also examined.
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[Journal Article] Creation of a type 1 blue copper site within a de novo coiled-coil protein scaffold2010
Author(s)
D.Shiga, D.Nakane, T.Inomata, Y.Funahashi, H.Masuda, A.Kikuchi, M.Oda, M.Noda, S.Uchiyama, K.Fukui, K.Kanaori, K.Tajima, Y.Takano, H.Nakamura, T.Tanaka
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Journal Title
J.Am.Chem.Soc. 132
Pages: 18191-18198
Peer Reviewed
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